Anti-MUC13 抗体 (ab65109)
Key features and details
- Rabbit polyclonal to MUC13
- Suitable for: ELISA, IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-MUC13 antibody
MUC13 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MUC13 -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide (Human) from an internal sequence.
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ポジティブ・コントロール
- WB: Extracts from COLO, 293 and HeLa cells. IHC-P: Human colon carcinoma tissue. IF: HepG2 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab65109の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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IHC-P | (1) |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
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ICC/IF |
1/500 - 1/1000.
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
ICC/IF
1/500 - 1/1000. |
ターゲット情報
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機能
Epithelial and hemopoietic transmembrane mucin that may play a role in cell signaling. -
組織特異性
Highly expressed in epithelial tissues, particularly those of the gastrointestinal and respiratory tracts, such as large intestine and trachea, followed by kidney, small intestine, appendix and stomach. -
配列類似性
Contains 3 EGF-like domains.
Contains 1 SEA domain. -
翻訳後修飾
Cleaved into two subunits, alpha and beta, probably between the first EGF domain and the SEA domain. Beta subunit contains the cytoplasmic tail and alpha subunit the extracellular tail. The homo-oligomerization into dimers is dependent on intrachain disulfide bonds.
Highly N-glycosylated. -
細胞内局在
Cell membrane. Secreted. Also exists as a soluble form. - Information by UniProt
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参照データベース
- Entrez Gene: 56667 Human
- Omim: 612181 Human
- SwissProt: Q9H3R2 Human
- Unigene: 5940 Human
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別名
- Down-regulated in colon cancer 1 antibody
- DRCC1 antibody
- FLJ20063 antibody
see all
画像
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Immunofluorescence analysis of HepG2 cells, using MUC13 antibody. The picture on the right is blocked with synthesized peptide.
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All lanes : Anti-MUC13 antibody (ab65109) at 1/500 dilution
Lane 1 : extracts from COLO cells
Lane 2 : extracts from 293 cells
Lane 3 : extracts from HeLa cells
Lane 4 : extracts from HeLa cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 55 kDa
Observed band size: 55 kDaSecondary antibody - anti-rabbit HRP (ab6721)
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Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labelling MUC13 with ab65109.
The picture on the right is blocked with the synthesized peptide. -
ICC/IF image of ab65109 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65109, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab65109 は 5 報の論文で使用されています。
- Pothuraju R et al. Depletion of transmembrane mucin 4 (Muc4) alters intestinal homeostasis in a genetically engineered mouse model of colorectal cancer. Aging (Albany NY) 14:2025-2046 (2022). PubMed: 35255004
- Lv J et al. Gasdermin E mediates resistance of pancreatic adenocarcinoma to enzymatic digestion through a YBX1-mucin pathway. Nat Cell Biol 24:364-372 (2022). PubMed: 35292781
- LaMonte GM et al. Dual RNA-seq identifies human mucosal immunity protein Mucin-13 as a hallmark of Plasmodium exoerythrocytic infection. Nat Commun 10:488 (2019). PubMed: 30700707
- Journo G et al. Modulation of Cellular CpG DNA Methylation by Kaposi's Sarcoma-Associated Herpesvirus. J Virol 92:N/A (2018). PubMed: 29899086
- Jepsen CS et al. FIBCD1 Binds Aspergillus fumigatus and Regulates Lung Epithelial Response to Cell Wall Components. Front Immunol 9:1967 (2018). PubMed: 30279687