製品の概要
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製品名Anti-MUC13 antibody
MUC13 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to MUC13
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由来種Rabbit
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アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
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種交差性交差種: Mouse, Human
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免疫原
A synthetic peptide from the C terminus of Human MUC13.
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ポジティブ･コントロール
- Human colon tissue; 293, HeLa and COLO205 cell lysates; HepG2 cells.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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バッファーpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
Note: PBS (without Mg2+, Ca2+) -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab124654 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/500 - 1/1999. Predicted molecular weight: 55 kDa. | |
| IHC-P | Use a concentration of 5 µg/ml. | |
| ICC/IF | 1/100 - 1/500. |
ターゲット情報
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機能Epithelial and hemopoietic transmembrane mucin that may play a role in cell signaling.
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組織特異性Highly expressed in epithelial tissues, particularly those of the gastrointestinal and respiratory tracts, such as large intestine and trachea, followed by kidney, small intestine, appendix and stomach.
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配列類似性Contains 3 EGF-like domains.
Contains 1 SEA domain. -
翻訳後修飾Cleaved into two subunits, alpha and beta, probably between the first EGF domain and the SEA domain. Beta subunit contains the cytoplasmic tail and alpha subunit the extracellular tail. The homo-oligomerization into dimers is dependent on intrachain disulfide bonds.
Highly N-glycosylated. -
細胞内局在Cell membrane. Secreted. Also exists as a soluble form.
- Information by UniProt
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参照データベース
- Entrez Gene: 56667 Human
- Entrez Gene: 17063 Mouse
- Omim: 612181 Human
- SwissProt: Q9H3R2 Human
- SwissProt: P19467 Mouse
- Unigene: 5940 Human
- Unigene: 3177 Mouse
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別名
- Down-regulated in colon cancer 1 antibody
- DRCC1 antibody
- FLJ20063 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody (ab124654)This image is courtesy of an Abreview submitted by Alessandro Gandelliab124654 staining MUC13 in mouse colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked for 30 minutes at room temperature. Samples were incubated with primary antibody (1/25 in PBS Tween) for 1 hour. An undiluted HRP polymer-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody (ab124654)ab124654, at 5 µg/ml, staining MUC13 in Formalin-fixed, Paraffin-embedded Human Colon tissue by Immunohistochemistry. -
Western blot - Anti-MUC13 antibody (ab124654)All lanes : Anti-MUC13 antibody (ab124654) at 1/500 dilution
Lane 1 : 293 cell lysate
Lane 2 : COLO205 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HeLa cell lysate with synthesized peptide
Predicted band size: 55 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-MUC13 antibody (ab124654)ab124654 at 1/100 dilution staining MUC13 in HepG2 cells by Immunofluorescence. The image on the right is treated with the synthesized peptide.
プロトコール
参考文献
This product has been referenced in:
- He L et al. Reduction of miR-132-3p contributes to gastric cancer proliferation by targeting MUC13. Mol Med Rep 15:3055-3061 (2017). WB, IHC-P ; Human . Read more (PubMed: 28339011) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"