製品の概要

  • 製品名Anti-MUC1 antibody
    MUC1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MUC1
  • アプリケーション適用あり: IHC-Fr, ICC/IF, IHC-Pmore details
    適用なし: WB
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (Human) from cytoplasmic tail.

  • ポジティブ・コントロール
    • Breast carcinoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/200.
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/100. Antigen retrieval is not essential but may optimise staining.

10min at RT.

  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 機能The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
      The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
    • 組織特異性Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform Y is expressed in tumor cells only.
    • 関連疾患MUC1/CA 15-3 is used as a serological clinical marker of breast cancer to monitor response to breast cancer treatment and disease recurrence (PubMed:20816948). Decreased levels over time may be indicative of a positive response to treatment. Conversely, increased levels may indicate disease progression. At an early stage disease, only 21% of patients exhibit high MUC1/CA 15-3 levels, that is why CA 15-3 is not a useful screening test. Most antibodies target the highly immunodominant core peptide domain of 20 amino acid (APDTRPAPGSTAPPAHGVTS) tandem repeats. Some antibodies recognize glycosylated epitopes.
      Medullary cystic kidney disease 1
    • 配列類似性Contains 1 SEA domain.
    • 発生段階During fetal development, expressed at low levels in the colonic epithelium from 13 weeks of gestation.
    • 翻訳後修飾Highly glycosylated (N- and O-linked carbohydrates and sialic acid). O-glycosylated to a varying degree on serine and threonine residues within each tandem repeat, ranging from mono- to penta-glycosylation. The average density ranges from about 50% in human milk to over 90% in T47D breast cancer cells. Further sialylation occurs during recycling. Membrane-shed glycoproteins from kidney and breast cancer cells have preferentially sialyated core 1 structures, while secreted forms from the same tissues display mainly core 2 structures. The O-glycosylated content is overlapping in both these tissues with terminal fucose and galactose, 2- and 3-linked galactose, 3- and 3,6-linked GalNAc-ol and 4-linked GlcNAc predominating. Differentially O-glycosylated in breast carcinomas with 3,4-linked GlcNAc. N-glycosylation consists of high-mannose, acidic complex-type and hybrid glycans in the secreted form MUC1/SEC, and neutral complex-type in the transmembrane form, MUC1/TM.
      Proteolytic cleavage in the SEA domain occurs in the endoplasmic reticulum by an autoproteolytic mechanism and requires the full-length SEA domain as well as requiring a Ser, Thr or Cys residue at the P + 1 site. Cleavage at this site also occurs on isoform MUC1/X but not on isoform MUC1/Y. Ectodomain shedding is mediated by ADAM17.
      Dual palmitoylation on cysteine residues in the CQC motif is required for recycling from endosomes back to the plasma membrane.
      Phosphorylated on tyrosines and serine residues in the C-terminal. Phosphorylation on tyrosines in the C-terminal increases the nuclear location of MUC1 and beta-catenin. Phosphorylation by PKC delta induces binding of MUC1 to beta-catenin/CTNNB1 and thus decreases the formation of the beta-catenin/E-cadherin complex. Src-mediated phosphorylation inhibits interaction with GSK3B. Src- and EGFR-mediated phosphorylation on Tyr-1229 increases binding to beta-catenin/CTNNB1. GSK3B-mediated phosphorylation on Ser-1227 decreases this interaction but restores the formation of the beta-cadherin/E-cadherin complex. On T-cell receptor activation, phosphorylated by LCK. PDGFR-mediated phosphorylation increases nuclear colocalization of MUC1CT and CTNNB1.
      The N-terminal sequence has been shown to begin at position 24 or 28.
    • 細胞内局在Secreted; Cell membrane. Cytoplasm. Nucleus. On EGF and PDGFRB stimulation, transported to the nucleus through interaction with CTNNB1, a process which is stimulated by phosphorylation. On HRG stimulation, colocalizes with JUP/gamma-catenin at the nucleus and Apical cell membrane. Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ADMCKD antibody
      • ADMCKD1 antibody
      • Breast carcinoma associated antigen DF3 antibody
      • Breast carcinoma-associated antigen DF3 antibody
      • CA 15-3 antibody
      • CA15 3 antibody
      • CA15 3 antigen antibody
      • CA15.3 antibody
      • Cancer antigen 15-3 antibody
      • Carcinoma associated mucin antibody
      • Carcinoma-associated mucin antibody
      • CD 227 antibody
      • CD227 antibody
      • DF3 antigen antibody
      • EMA antibody
      • Episialin antibody
      • H23 antigen antibody
      • H23AG antibody
      • KL 6 antibody
      • KL-6 antibody
      • KL6 antibody
      • Krebs von den Lungen-6 antibody
      • MAM 6 antibody
      • MAM6 antibody
      • MCD antibody
      • MCKD antibody
      • MCKD1 antibody
      • Medullary cystic kidney disease 1 (autosomal dominant) antibody
      • Medullary cystic kidney disease, autosomal dominant antibody
      • MUC 1 antibody
      • MUC-1 antibody
      • MUC-1/SEC antibody
      • MUC-1/X antibody
      • MUC1 antibody
      • MUC1-alpha antibody
      • MUC1-beta antibody
      • MUC1-CT antibody
      • MUC1-NT antibody
      • MUC1/ZD antibody
      • MUC1_HUMAN antibody
      • Mucin 1 antibody
      • Mucin 1 transmembrane antibody
      • Mucin 1, cell surface associated antibody
      • Mucin-1 subunit beta antibody
      • Peanut reactive urinary mucin antibody
      • Peanut-reactive urinary mucin antibody
      • PEM antibody
      • PEMT antibody
      • Polymorphic epithelial mucin antibody
      • PUM antibody
      • Tumor associated epithelial membrane antigen antibody
      • Tumor associated epithelial mucin antibody
      • Tumor associated mucin antibody
      • Tumor-associated epithelial membrane antigen antibody
      • Tumor-associated mucin antibody
      see all

    Anti-MUC1 antibody 画像

    • Formalin fixed paraffin embedded breast carcinoma stained with anti-MUC1 antibody, ab15481.
    • ab15481 at 1/200 staining mouse mammary gland tissue sections by IHC-P. The tissue was paraformaldehyde fixed and blocked with BSA. A heat mediated antigen retrieval step was performed. The antibody was incubated with the tissue for 16 hours and then an Alexa-Fluor 488 conjugated goat anti-rabbit antibody was used as the secondary. MUC1 staining (luminal cells) is shown in green. The nuclei were counterstained with DAPI and staining is shown in blue.

      See Abreview

    • ICC/IF image of ab15481 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15481, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Anti-MUC1 antibody (ab15481) 使用論文

    This product has been referenced in:
    • Prater MD  et al. Mammary stem cells have myoepithelial cell properties. Nat Cell Biol 16:942-50 (2014). IHC-P ; Mouse . Read more (PubMed: 25173976) »
    • Tezval H  et al. Reduced mrna expression level of corticotropin-releasing hormone-binding protein is associated with aggressive human kidney cancer. BMC Cancer 13:199 (2013). IHC-P ; Human . Read more (PubMed: 23607589) »

    See all 12 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Virgin tissue)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate 95C
    Permeabilization Yes - PBS with 3% Triton
    Specification Virgin tissue
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative Formaldehyde
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    投稿 Aug 03 2015

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Breast -MCF7)
    Permeabilization Yes - 0.25% Triton, 15 min
    Specification Breast -MCF7
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative Paraformaldehyde
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    Abcam user community

    Verified customer

    投稿 Jul 22 2015

    Thank you for contacting Abcam.
    I am sorry about the issues you have been having with ab15481 in IHC-P. As we discussed I am sending you a new vial of this antibody from a different lot as a free of charge replacement. Your new order number is ***...

    Read More

    Sorry for the delay in contacting you with regards to this query. We have a number of pairs of mouse monoclonals which can be used to detect the under or unglycosylated form of MUC1 (for example ab10117 which can be paired with HRP conjugated ab24471)....

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Mammary gland)
    Specification Mammary gland
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH6
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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    Verified customer

    投稿 Nov 17 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Pancreas)
    Specification Pancreas
    Fixative Formaldehyde
    Permeabilization No
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 22°C
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    Verified customer

    投稿 Jan 25 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Mouse Nasal Epithelium)
    Specification Mouse Nasal Epithelium
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated
    Permeabilization No
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10%
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    Verified customer

    投稿 Jan 02 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (mammary gland)
    Specification mammary gland
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
    Username

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    Verified customer

    投稿 Mar 09 2007

    Thank you for your enquiry regarding our MUC1 antibodies. There is indeed a large selection of those antibodies available in our catalogue and I have summarised below the information we have regarding the epitope recognised and whether we know if th...

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    The BSA concentration is 0.2mg/ml.

    1-10 of 12 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"