Anti-Mu Opioid Receptor 抗体 [mAb63256] (ab63256)

製品の概要

  • 製品名
    Anti-Mu Opioid Receptor antibody [mAb63256]
    Mu Opioid Receptor 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [mAb63256] to Mu Opioid Receptor
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Horse, Cow, Human, Non human primates
  • 免疫原

    Synthetic peptide corresponding to Human Mu Opioid Receptor aa 300 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab98257, ab98257, ab98256)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in mouse spinal cord tissue lysate.
  • 特記事項

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab63256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 10 µg/ml. Detects a band of approximately 45, 51, 58 kDa (predicted molecular weight: 45 kDa).

ターゲット情報

画像

  • Anti-Mu Opioid Receptor antibody [mAb63256] (ab63256) at 10 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 20 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
    Additional bands at : 13.5 kDa,51 kDa (possible glycosylated form),58 kDa (possible glycosylated form). We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

プロトコール

参考文献

ab63256 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Chicken Tissue lysate - whole (forebrain)
Gel Running Conditions
Non-reduced Denaturing (Biorad Mini-Protean Precast gel. 4-20%)
Loading amount
25 µg
Specification
forebrain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Mussie Ghezu Hadera

Verified customer

投稿 Dec 19 2017



Ich stimme Ihnen zu, dass das Protokoll etwas umständlich geschrieben ist.

Bitte setzen Sie doppelt so viel Peptid wie Antikörper ein:

Antikörper: 2 ug/ml (5ml Lösung: 10 ug Antikörper)

B...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

投稿 Mar 20 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録