製品の概要

  • 製品名
    Anti-Mu Opioid Receptor antibody
    Mu Opioid Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Mu Opioid Receptor
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Fr, ICC, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Guinea pig, Human
    交差が予測される動物種: Cow, Pig, Non human primates, Macaque monkey
  • 免疫原

    Synthetic peptide:

    NHQLENLEAETAPLP

    , corresponding to C terminal amino acids 384-398 of Rat Mu Opioid Receptor 1.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100 - 1/2000.
WB 1/500 - 1/1000. Detects a band of approximately 49-65 kDa (predicted molecular weight: 45 kDa).Can be blocked with Rat Mu Opioid Receptor peptide (ab46988).

Approximately 49 -65 kDa depending on the tissue lysate. Can be blocked with ab46988.

IHC-Fr 1/100 - 1/2000. We recommend incubating for 18-24 hours at 4C.
ICC 1/100 - 1/2000.
IHC-FoFr 1/100 - 1/2000. We recommend incubating for 18-24 hours at 4C.

ターゲット情報

画像

  • Ab10275 (1:2500) staining of Mu opioid receptor by IHC.
  • ICC/IF image of ab10275 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10275, 1 in 200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of rat brain tissue, staining Mu Opioid Receptor with ab10275.

    Tissue was blocked with 5% normal donkey serum and 0.4% Triton X-100 for 1 hour at room temperature. Sections were incubated with primary antibody (1/2000) for 24 hours at 4°C. An AlexaFluor®594-conjugated donkey anti-rabbit IgG (1/250) was used as the secondary antibody.
  • Mu Opioid Receptor (ab10275) staining of Rat DRG (dilution: 1:100) incubation at 4 °C overnight. Secondary antibody is anti-Rabbit Rhodamine Red (dilution:1:200) incubation at room temperature for 1 hour.

参考文献

This product has been referenced in:
  • Song C & Xue L Roles of the µ-opioid receptor and its related signaling pathways in the pathogenesis of premenstrual syndrome liver-qi stagnation. Exp Ther Med 13:3130-3136 (2017). Read more (PubMed: 28587388) »
  • Yang Z  et al. Reverse of Acute and Chronic Morphine Tolerance by Lithocholic Acid via Down-Regulating UGT2B7. Front Pharmacol 7:404 (2016). WB ; Mouse . Read more (PubMed: 27847477) »

See all 23 Publications for this product

レビューと Q&A

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse, whole brain sections)
Antigen retrieval step
None
Permeabilization
Yes - 10% Tween-20
Specification
Mouse, whole brain sections
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

投稿 Mar 14 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - other (Gross brain dissected tissue)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Gross brain dissected tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jul 26 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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