ICC/IF image of ab10275 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10275, 1 in 200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Mu Opioid Receptor antibody (ab10275)Image from Jedynak JP et al., PLoS One. 2012;7(4):e34227. Epub 2012 Apr 13. Fig 2.; doi:10.1371/journal.pone.0034227; April 13, 2012, PLoS ONE 7(4): e34227.
Immunohistochemical analysis of rat brain tissue, staining Mu Opioid Receptor with ab10275.
Tissue was blocked with 5% normal donkey serum and 0.4% Triton X-100 for 1 hour at room temperature. Sections were incubated with primary antibody (1/2000) for 24 hours at 4°C. An AlexaFluor®594-conjugated donkey anti-rabbit IgG (1/250) was used as the secondary antibody.
Mu Opioid Receptor (ab10275) staining of Rat DRG (dilution: 1:100) incubation at 4 °C overnight. Secondary antibody is anti-Rabbit Rhodamine Red (dilution:1:200) incubation at room temperature for 1 hour.
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