製品の概要

  • 製品名Anti-Mu Opioid Receptor antibody
    Mu Opioid Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Mu Opioid Receptor
  • アプリケーション適用あり: ICC/IF, WB, IHC-Fr, ICC, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Guinea pig, Human
    交差が予測される動物種: Cow, Pig, Non Human Primates, Macaque Monkey
  • 免疫原

    Synthetic peptide:

    NHQLENLEAETAPLP

    , corresponding to C terminal amino acids 384-398 of Rat Mu Opioid Receptor 1.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100 - 1/2000.
WB 1/500 - 1/1000. Detects a band of approximately 49-65 kDa (predicted molecular weight: 45 kDa).Can be blocked with Rat Mu Opioid Receptor peptide (ab46988).

Approximately 49 -65 kDa depending on the tissue lysate. Can be blocked with ab46988.

IHC-Fr 1/100 - 1/2000. We recommend incubating for 18-24 hours at 4C.
ICC 1/100 - 1/2000.
IHC-FoFr 1/100 - 1/2000. We recommend incubating for 18-24 hours at 4C.

ターゲット情報

Anti-Mu Opioid Receptor antibody 画像

  • Ab10275 (1:2500) staining of Mu opioid receptor by IHC.
  • ICC/IF image of ab10275 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10275, 1 in 200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of rat brain tissue, staining Mu Opioid Receptor with ab10275.

    Tissue was blocked with 5% normal donkey serum and 0.4% Triton X-100 for 1 hour at room temperature. Sections were incubated with primary antibody (1/2000) for 24 hours at 4°C. An AlexaFluor®594-conjugated donkey anti-rabbit IgG (1/250) was used as the secondary antibody.
  • Mu Opioid Receptor (ab10275) staining of Rat DRG (dilution: 1:100) incubation at 4 °C overnight. Secondary antibody is anti-Rabbit Rhodamine Red (dilution:1:200) incubation at room temperature for 1 hour.

Anti-Mu Opioid Receptor antibody (ab10275) 使用論文

This product has been referenced in:
  • Lantero A  et al. TGF-ß and opioid receptor signaling crosstalk results in improvement of endogenous and exogenous opioid analgesia under pathological pain conditions. J Neurosci 34:5385-95 (2014). Mouse . Read more (PubMed: 24719115) »
  • Brewer KL  et al. Dopamine D3 receptor dysfunction prevents anti-nociceptive effects of morphine in the spinal cord. Front Neural Circuits 8:62 (2014). WB ; Mouse . Read more (PubMed: 24966815) »

See all 15 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - other (Gross brain dissected tissue)
Gel Running Conditions Reduced Denaturing
Loading amount 30 µg
Specification Gross brain dissected tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Jul 26 2016

Gracias por contactarnos.
Después de consultar este caso con mis compañeros del laboratorio, acerca de los siguientes anticuerpos contra Mu Opioid Receptor validados en tejidos de rata: ab10275, ab17934, ab64746, ab128013 y ab134054, me temo que n...

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Thank you for your message and for inform us about this problem.

I am sorry to hear that the free of charge replacement has not improved the results on this occasion. Due to your valuable information this antibody will now be further investiga...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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I am sorry to confirm that we don't have any different lots in stock at the moment. I would like to reassure you that we monitor feedback closely on a weekly basis and we are not currently concerned about the general...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this c...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from our Anti-Mu Opioid Receptor antibody (ab10275).

I would like to reassure you that this antibody is tested and covered b...

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I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the origi...

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Thanks for your enquiry.

ab10275 is a whole antiserum product and the exact concentration of the anti-Mu opioid receptor in the serum has not been determined. In general, however, specific antibody concentration inproducts of this type is typ...

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Thank you for calling Abcam earlier today.

I am sorry that you have been having issues with ab10275 in IHC-Fr on mouse brain tissue sections. As we discussed on the telephone, I am sending you a new vial of the antibody (from a different lot) ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"