製品の概要

  • 製品名Anti-mu Crystallin antibody
    mu Crystallin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to mu Crystallin
  • アプリケーション適用あり: WB, ICC/IF, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment corresponding to Human mu Crystallin aa 215-314.
    Sequence:

    EWVKPGAHINAVGASRPDWRELDDELMKEAVLYVDSQEAALKESGDVLLS GAEIFAELGEVIKGVKPAHCEKTTVFKSLGMAVEDTVAAKLIYDSWSSGK

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab54669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 34 kDa.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Binds thyroid hormone. Presumably involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptors.
  • 組織特異性Expressed in neural tissue, muscle and kidney.
  • 配列類似性Belongs to the ornithine cyclodeaminase family.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CRYM antibody
    • CRYM_HUMAN antibody
    • Crystallin mu antibody
    • DFNA 40 antibody
    • DFNA40 antibody
    • Ketimine reductase antibody
    • Mu crystallin homolog antibody
    • Mu-crystallin homolog antibody
    • NADP regulated thyroid hormone binding protein antibody
    • NADP-regulated thyroid-hormone-binding protein antibody
    • OTTHUMP00000115878 antibody
    • THBP antibody
    • Thiomorpholine carboxylate dehydrogenase antibody
    see all

Anti-mu Crystallin antibody 画像



  • Predicted band size : 34 kDa
    mu Crystallin antibody (ab54669) at 1ug/lane + Jurkat cell lysate at 25ug/lane.
  • ICC/IF image of ab54669 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54669, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • mu Crystallin antibody (ab54669) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human skeletal muscle.
  • Overlay histogram showing SH-SY5Y cells stained with ab54669 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54669, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-mu Crystallin antibody (ab54669) 使用論文

This product has been referenced in:
  • Zuccotti A  et al. The transcription factor Fezf2 directs the differentiation of neural stem cells in the subventricular zone toward a cortical phenotype. Proc Natl Acad Sci U S A 111:10726-31 (2014). Read more (PubMed: 25002477) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (mouse retina whole cell lysate)
Loading amount 30 µg
Specification mouse retina whole cell lysate
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Nov 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"