製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MTTP
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Human MTTP.

    (Peptide available as ab99872.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Liver Tissue Lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab63467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).

ターゲット情報

  • 機能
    Catalyzes the transport of triglyceride, cholesteryl ester, and phospholipid between phospholipid surfaces. Required for the secretion of plasma lipoproteins that contain apolipoprotein B.
  • 組織特異性
    Liver and small intestine. Also found in ovary, testis and kidney.
  • 関連疾患
    Abetalipoproteinemia
  • 配列類似性
    Contains 1 vitellogenin domain.
  • 細胞内局在
    Endoplasmic reticulum.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ABL antibody
    • Microsomal triglyceride transfer protein (large polypeptide 88kDa) antibody
    • Microsomal triglyceride transfer protein 88kD antibody
    • Microsomal triglyceride transfer protein antibody
    • Microsomal triglyceride transfer protein large subunit antibody
    • MTP antibody
    • MTP large subunit antibody
    • MTP_HUMAN antibody
    • MTTP antibody
    • Transfer RNA, mitochondrial, proline antibody
    • tRNA-Pro, mitochondrial antibody
    see all

画像

  • Anti-MTTP antibody (ab63467) at 1 µg/ml + Liver (Human) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 99 kDa
    Observed band size : 99 kDa


    Exposure time : 20 minutes
  • ICC/IF image of ab63467 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab63467 at 1µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5min) HeLa cell types at 1ug/ml

参考文献

This product has been referenced in:
  • Yamamoto Y  et al. Rapid alternative absorption of dietary long-chain fatty acids with upregulation of intestinal glycosylated CD36 in liver cirrhosis. Am J Clin Nutr 96:90-101 (2012). Human . Read more (PubMed: 22648712) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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