製品の概要

  • 製品名Anti-mtTFA antibody
    mtTFA 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to mtTFA
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1 - 246 of Human mtTFA (NP_003192.1)

  • ポジティブ・コントロール
    • WB: kidney lysate. IHC: small intestine tissue. ICC/IF: HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab89818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
ICC/IF
  • 追加情報ICC/IF: Use at a concentration of 10 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimize staining. Microwave method recommended.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M.
    • 配列類似性Contains 2 HMG box DNA-binding domains.
    • 細胞内局在Mitochondrion.
    • Information by UniProt
    • 参照データベース
    • 別名
      • anscription factor 6-like 1 antibody
      • Mitochondrial transcription factor 1 antibody
      • mitochondrial transcription factor A antibody
      • MtTF1 antibody
      • mtTFA antibody
      • TCF 6 antibody
      • TCF-6 antibody
      • TCF6 antibody
      • TCF6L1 antibody
      • TCF6L2 antibody
      • TCF6L3 antibody
      • TFAM antibody
      • TFAM_HUMAN antibody
      • Transcription factor 6 antibody
      • Transcription factor 6 like 2 (mitochondrial transcription factor) antibody
      • Transcription factor 6 like 2 antibody
      • Transcription factor 6-like 2 antibody
      • transcription factor 6-like 3 antibody
      • Transcription factor A, mitochondrial antibody
      • Transcription factor A, mitochondrial antibody
      • Transcription factor A, mitochondrial precursor antibody
      see all

    Anti-mtTFA antibody 画像

    • Anti-mtTFA antibody (ab89818) at 1 µg/ml + Human kidney lysate at 50 µg

      Predicted band size : 29 kDa
    • All lanes : Anti-mtTFA antibody (ab89818) at 1 µg/ml

      Lane 1 : mtTFA transfected 293T cell lysate
      Lane 2 : Non-transfected lysate

      Lysates/proteins at 25 µg per lane.


      Predicted band size : 29 kDa
      Observed band size : 29 kDa
      Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
    • ab89818 at 3µg/ml staining mtTFA in formalin-fixed, paraffin-embedded Human small intestine tissue by immunohistochemistry.
    • ab89818 at 10µg/ml staining mtTFA in PFA-fixed, permeabilized HeLa cells by immunofluorescence.

    Anti-mtTFA antibody (ab89818) 使用論文

    This product has been referenced in:
    • Riley LG  et al. Phenotypic variability and identification of novel YARS2 mutations in YARS2 mitochondrial myopathy, lactic acidosis and sideroblastic anaemia. Orphanet J Rare Dis 8:193 (2013). WB . Read more (PubMed: 24344687) »
    • Berquist BR  et al. Human Cockayne syndrome B protein reciprocally communicates with mitochondrial proteins and promotes transcriptional elongation. Nucleic Acids Res 40:8392-405 (2012). Read more (PubMed: 22743267) »

    See all 4 Publications for this product

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    There are currently no Abreviews or Questions for ab89818.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"