Anti-mtTFA 抗体 [18G102B2E11] - Mitochondrial Marker (ab119684)
Key features and details
- Mouse monoclonal [18G102B2E11] to mtTFA - Mitochondrial Marker
- Suitable for: IHC-P, WB, Flow Cyt, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2b
Related conjugates and formulations
製品の概要
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製品名
Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker
mtTFA 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [18G102B2E11] to mtTFA - Mitochondrial Marker -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, WB, Flow Cyt, ICC/IFmore details -
種交差性
交差種: Human, Recombinant fragment
非交差種: Mouse, Rat -
免疫原
Full length protein. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IHC-P: human liver FFPE tissue sections
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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精製度
Ammonium Sulphate Precipitation -
特記事項(精製)
Purity is near homogeneity as judged by SDS-PAGE. ab119684 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. -
ポリ/モノ
モノクローナル -
クローン名
18G102B2E11 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab119684の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
Use a concentration of 1 µg/ml.
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特記事項 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M. -
配列類似性
Contains 2 HMG box DNA-binding domains. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 7019 Human
- Omim: 600438 Human
- SwissProt: Q00059 Human
- Unigene: 642966 Human
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別名
- anscription factor 6-like 1 antibody
- Mitochondrial transcription factor 1 antibody
- mitochondrial transcription factor A antibody
see all
画像
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All lanes : Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker (ab119684) at 1 µg/ml
Lane 1 : recombinant mtTFA (ab103784) at 0.025 µg
Lane 2 : HeLa whole cell lysate at 30 µg
Secondary
All lanes : HRP-conjugated Goat polyclonal to mouse IgG at 1/5000 dilution
Predicted band size: 29 kDa -
Immunocytochemistry image of ab119684 stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (18G102B2E11, 1µg/ml) for 2h at room temperature. The secondary antibody was (green) 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). mtTFA localizes to the mitochondria.
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HeLa cells were stained with 2 µg/mL mtTFA antibody (ab119684) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
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IHC image of mtTFA staining in human liver HCC formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab119684, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (23)
ab119684 は 23 報の論文で使用されています。
- Alexander JF et al. Targeting the Meningeal Compartment to Resolve Chemobrain and Neuropathy via Nasal Delivery of Functionalized Mitochondria. Adv Healthc Mater 11:e2102153 (2022). PubMed: 35007407
- Alexander JF et al. Nasal administration of mitochondria reverses chemotherapy-induced cognitive deficits. Theranostics 11:3109-3130 (2021). PubMed: 33537077
- Stuani L et al. Mitochondrial metabolism supports resistance to IDH mutant inhibitors in acute myeloid leukemia. J Exp Med 218:N/A (2021). PubMed: 33760042
- Hao Z et al. N6-Deoxyadenosine Methylation in Mammalian Mitochondrial DNA. Mol Cell 78:382-395.e8 (2020). PubMed: 32183942
- Maekawa H et al. Mitochondrial Damage Causes Inflammation via cGAS-STING Signaling in Acute Kidney Injury. Cell Rep 29:1261-1273.e6 (2019). PubMed: 31665638