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Our Abpromise guarantee covers the use of ab25880 in the following tested applications.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 2 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 289 kDa.Can be blocked with Human mTOR peptide (ab99155).|
|IP||Use at an assay dependent concentration.|
IHC image of mTOR staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25880, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab25880 at 2µg/ml staining mTOR in L1210 cells by ICC/IF
ab25880 has not yet been referenced specifically in any publications.