Anti-MTMR14 抗体 (ab102575)
Key features and details
- Rabbit polyclonal to MTMR14
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MTMR14 antibody
MTMR14 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MTMR14 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 264 and 415 of Human MTMR14 (AAH01674; UniProt ID: Q8NCE2 isoform 3).
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ポジティブ・コントロール
- WB: 293T, A431, Jurkat and Raji cell lysates IHC-P: MCF7 xenograft
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab102575の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 72 kDa.
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IHC-P |
1/100 - 1/500. Antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0) is recommended.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 72 kDa. |
IHC-P
1/100 - 1/500. Antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0) is recommended. |
ターゲット情報
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機能
Lipid phosphatase which efficiently dephosphorylates phosphatidylinositol 3-phosphate (PtdIns3P) and PtdIns(3,5)P2; inactive toward PtdIns4P, PtdIns(3,4)P2, PtdIns(4,5)P2 and PtdIns(3,4,5)P3. -
組織特異性
Expressed in various tissues, including heart, skeletal muscle, placenta, liver, lung, kidney and pancreas. -
関連疾患
Defects in MTMR14 may be a cause of centronuclear myopathy autosomal dominant (ADCNM) [MIM:160150]; also known as autosomal dominant myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers. -
配列類似性
Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily. -
細胞内局在
Cytoplasm. Found in reticular structures and plasma membrane ruffles. Concentrated near the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 64419 Human
- Entrez Gene: 97287 Mouse
- Entrez Gene: 312634 Rat
- Omim: 611089 Human
- SwissProt: Q8NCE2 Human
- SwissProt: Q8VEL2 Mouse
- Unigene: 475382 Human
- Unigene: 197816 Mouse
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別名
- C3orf29 antibody
- Egg derived tyrosine phosphatase homolog antibody
- FLJ11546 antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab102575 は 4 報の論文で使用されています。
- Kong LY et al. Myotubularin-Related Protein14 Prevents Neointima Formation and Vascular Smooth Muscle Cell Proliferation by Inhibiting Polo-Like Kinase1. J Am Heart Assoc 11:e026174 (2022). PubMed: 36314496
- Kovács T et al. A conserved MTMR lipid phosphatase increasingly suppresses autophagy in brain neurons during aging. Sci Rep 12:21817 (2022). PubMed: 36528685
- Wen N et al. Deficiency of MTMR14 impairs male fertility in Mus musculus. PLoS One 13:e0206224 (2018). PubMed: 30412589
- Kovács T et al. The small molecule AUTEN-99 (autophagy enhancer-99) prevents the progression of neurodegenerative symptoms. Sci Rep 7:42014 (2017). IHC . PubMed: 28205624