製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to MTM1
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Cow, Dog
  • 免疫原

    Synthetic peptide:

    SSPSQMMPHVQTHF

    from C Terminus of the protein sequence according to NP_000243.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
ELISA 1/16000.

ターゲット情報

  • 機能
    Lipid phosphatase which dephosphorylates phosphatidylinositol 3-monophosphate (PI3P) and phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2). Has also been shown to dephosphorylate phosphotyrosine- and phosphoserine-containing peptides. Negatively regulates EGFR degradation through regulation of EGFR trafficking from the late endosome to the lysosome. Plays a role in vacuolar formation and morphology. Regulates desmin intermediate filament assembly and architecture. Plays a role in mitochondrial morphology and positioning. Required for skeletal muscle maintenance but not for myogenesis.
  • 関連疾患
    Defects in MTM1 are the cause of centronuclear myopathy X-linked (XCNM) [MIM:310400]; also known as X-linked myotubular myopathy (XLMTM) or myotubular myopathy type 1 (MTM1). Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.
  • 配列類似性
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.
    Contains 1 GRAM domain.
    Contains 1 myotubularin phosphatase domain.
  • ドメイン
    The GRAM domain mediates binding to PI(3,5)P2 and, with lower affinity, to other phosphoinositides.
  • 細胞内局在
    Cytoplasm. Cell membrane. Cell projection > filopodium. Cell projection > ruffle. Late endosome. Localizes as a dense cytoplasmic network. Also localizes to the plasma membrane, including plasma membrane extensions such as filopodia and ruffles. Predominantly located in the cytoplasm following interaction with MTMR12. Recruited to the late endosome following EGF stimulation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AF073996 antibody
    • CG2 antibody
    • CNM antibody
    • KIAA4176 antibody
    • mKIAA4176 antibody
    • Mtm antibody
    • Mtm1 antibody
    • MTM1_HUMAN antibody
    • MTMX antibody
    • Myotubular myopathy 1 antibody
    • Myotubularin antibody
    • XLMTM antibody
    see all

画像

  • Anti-MTM1 antibody (ab1350) at 1 µg/ml + HepG2 cell lysate (35µg protein in RIPA buffer)

    Predicted band size : 70 kDa

    Primary incubation was 1 hour. Detected by chemiluminescence.

  • Anti-MTM1 antibody (ab1350) at 1 µg/ml + Mouse Skeletal Muscle lysate (35µg protein in RIPA buffer)

    Predicted band size : 70 kDa

    Primary incubation was 1 hour. Detected by chemiluminescence.

  • Anti-MTM1 antibody (ab1350) at 1 µg/ml + Human Skeletal Muscle lysate (35µg protein in RIPA buffer)

    Predicted band size : 70 kDa

    Primary incubation was 1 hour. Detected by chemiluminescence.

プロトコール

参考文献

ab1350 has not yet been referenced specifically in any publications.

レビューと Q&A

This antibody is supplied as 100 ug. In Western blotting 1 ug/ml is a common concentration, therefore you will get approximately 100 Western blots. Use 20 ug of sample per lane.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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