Anti-MTHFS 抗体 (ab104009)
Key features and details
- Rabbit polyclonal to MTHFS
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MTHFS antibody -
製品の詳細
Rabbit polyclonal to MTHFS -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow -
免疫原
Recombinant fragment, corresponding to a region within amino acids 57-203 of Human MTHFS (AAH19921).
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ポジティブ・コントロール
- A549 and HepG2 whole cell lysates; AGS xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab104009の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 23 kDa.
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IHC-P |
1/100 - 1/500. (Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (PH8.0)).
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 23 kDa. |
IHC-P
1/100 - 1/500. (Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (PH8.0)). |
ターゲット情報
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機能
Contributes to tetrahydrofolate metabolism. Helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids. Catalyzes the irreversible conversion of 5-formyltetrahydrofolate (5-FTHF) to yield 5,10-methenyltetrahydrofolate. -
配列類似性
Belongs to the 5-formyltetrahydrofolate cyclo-ligase family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 513372 Cow
- Entrez Gene: 10588 Human
- Entrez Gene: 107885 Mouse
- Entrez Gene: 300886 Rat
- Omim: 604197 Human
- SwissProt: P49914 Human
- SwissProt: Q9D110 Mouse
- Unigene: 194294 Human
see all -
別名
- 10-methenyl-tetrahydrofolate synthetase antibody
- 5 10 methenyl tetrahydrofolate synthetase antibody
- 5 antibody
see all
画像
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All lanes : Anti-MTHFS antibody (ab104009) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 23 kDa
12% SDS-PAGE. -
ab104009 at 1/100 dilution staining MTHFS in AGS xenograft by Immunohistochemistry, Paraffin-embedded tissue.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab104009 は論文での使用が確認できていません。