製品の概要

  • 製品名Anti-MTFMT antibody
  • 製品の詳細
    Mouse polyclonal to MTFMT
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    MTFMT (NP_640335.1, 1 a.a. ~ 304 a.a) full-length human protein.

  • ポジティブ・コントロール
    • MTFMT transfected 293T lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 44 kDa).

ターゲット情報

  • 機能Formylates methionyl-tRNA in mitochondria. A single tRNA(Met) gene gives rise to both an initiator and an elongator species via an unknown mechanism.
  • 配列類似性Belongs to the fmt family.
  • ドメインComposed of an N- and a C-terminal domain. The N-terminal domain carries the tetrahydrofolate (THF)-binding site and the C-terminal domain is presumably involved in positioning the Met-tRNA substrate for the formylation reaction.
  • 細胞内局在Mitochondrion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FMT antibody
    • FMT_HUMAN antibody
    • FMT1 antibody
    • Methionyl tRNA formyltransferase, mitochondrial antibody
    • Methionyl-tRNA formyltransferase antibody
    • mitochondrial antibody
    • mitochondrial methionyl tRNA formyltransferase antibody
    • MtFMT antibody
    see all

Anti-MTFMT antibody 画像

  • All lanes : Anti-MTFMT antibody (ab70100) at 1/500 dilution

    Lane 1 : MTFMT transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    MTFMT transfected lysate at 1/2500 dilution

    Predicted band size : 44 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)

Anti-MTFMT antibody (ab70100) 使用論文

ab70100 has not yet been referenced specifically in any publications.

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ab70100: It has been tested in Western blotin MTFMT transfected 293T cell line as the WB image indicates. We did not get any signal on non-transfected 293T lysate. I can confirm th...

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