製品の概要

  • 製品名
  • 製品の詳細
    Mouse monoclonal to MTF1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: GTVYDRTTVL IEQDPGTLED EDDDGQCGEH LPFLVGGEEG FHLIDHEAMS QGYVQHIISP DQIHLTINPG STPMPRNIEG ATLTLQSECP ETKRKEVKRY , corresponding to amino acids 41-141 of Human MTF1

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • アイソタイプ
    IgG2a
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab55522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml.

Please see specific references PMID: 22021037 and 18990686 regarding use of this antibody to detect endogenous human MTF1.

ターゲット情報

  • 機能
    Activates the metallothionein I promoter. Binds to the metal responsive element (MRE).
  • 配列類似性
    Contains 6 C2H2-type zinc fingers.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Metal regulatory transcription factor 1 antibody
    • Metal response element binding transcription factor 1 antibody
    • Metal responsive transcription factor 1 antibody
    • MRE binding transcription factor antibody
    • MRE-binding transcription factor 1 antibody
    • MRE-binding transcription factor antibody
    • MTF 1 antibody
    • MTF1 antibody
    • MTF1_HUMAN antibody
    • Transcription factor MTF-1 antibody
    • Transcription factor MTF1 antibody
    • Zinc regulatory factor antibody
    • ZRF antibody
    see all

Anti-MTF1 antibody 画像

  • Western blot against tagged recombinant protein immunogen using ab55522 MTF1 antibody at 1ug/ml. Predicted band size of the recombinant immunogen is 37 kDa.
    We have only tested this antibody against the reecombinant protein.
    Please see specific references PMID: 22021037 and 18990686 regarding use of this antibody to detect endogenous human MTF1.

プロトコール

Anti-MTF1 antibody (ab55522) 使用論文

This product has been referenced in:
  • Liu YC  et al. The Role of Small Ubiquitin-like Modifier-interacting Motif in the Assembly and Regulation of Metal-responsive Transcription Factor 1. J Biol Chem 286:42818-29 (2011). WB ; Human . Read more (PubMed: 22021037) »
  • Bellingham SA  et al. Regulation of prion gene expression by transcription factors SP1 and metal transcription factor-1. J Biol Chem 284:1291-301 (2009). WB ; Human . Read more (PubMed: 18990686) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (human MDM)
Loading amount
100 µg
Specification
human MDM
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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投稿 Jan 28 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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