Anti-MTCO2 抗体 [EPR3313] (ab109739)


  • 製品名Anti-MTCO2 antibody [EPR3313]
    MTCO2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3313] to MTCO2
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
    適用なし: Flow Cyt or ICC
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Cytochrome C oxidase subunit II.

  • ポジティブ・コントロール
    • HeLa, K562, MCF7, and THP1 cell lysates; Human kidney and liver tissues.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab109739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Antigen retrieval recommended; heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • 追加情報Is unsuitable for Flow Cyt or ICC.
  • ターゲット情報

    • 機能Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
    • 関連疾患Defects in MT-CO2 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
    • 配列類似性Belongs to the cytochrome c oxidase subunit 2 family.
    • 細胞内局在Mitochondrion inner membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • COII antibody
      • COX2 antibody
      • COX2_HUMAN antibody
      • COXII antibody
      • Cytochrome c oxidase II antibody
      • Cytochrome c oxidase polypeptide II antibody
      • Cytochrome c oxidase subunit 2 antibody
      • MT CO2 antibody
      • MT-CO2 antibody
      • MTCO2 antibody
      see all

    Anti-MTCO2 antibody [EPR3313] 画像

    • All lanes : Anti-MTCO2 antibody [EPR3313] (ab109739) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : THP1 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 26 kDa
    • ab109739 at 1/100 dilution staining Cytochrome C oxidase subunit II in paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • ab109739 at 1/100 dilution staining Cytochrome C oxidase subunit II in paraffin-embedded Human liver tissue by Immunohistochemistry.

    Anti-MTCO2 antibody [EPR3313] (ab109739) 使用論文

    ab109739 has not yet been referenced specifically in any publications.

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    The immunogens are as follows;

    ab79393; A synthetic peptide corresponding to residues in human Cytochrome C oxidase subunit II
    ab109739; A synthetic peptide corresponding to residues in Human Cytochro...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa WT vs HeLa rho-0)
    Loading amount 13 µg
    Specification HeLa WT vs HeLa rho-0
    Gel Running Conditions Reduced Denaturing (Reduced Denaturing (4-15%))
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

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    投稿 Jul 27 2012