製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MTAP
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 253 of MTAP (NP_002442)

  • ポジティブ・コントロール
    • 293T, H1299, HeLa, HepG2, Molt-4 or Raji cell lysate. A431 whole cell lysate. SAS xenograft tissue

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab96231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 31 kDa.
IHC-P 1/100 - 1/500.

ターゲット情報

  • 機能
    Plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine.
  • 組織特異性
    Ubiquitously expressed.
  • 配列類似性
    Belongs to the PNP/MTAP phosphorylase family.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5' methylthioadenosine phosphorylase antibody
    • 5''-methylthioadenosine phosphorylase antibody
    • BDMF antibody
    • c86fus antibody
    • DMSFH antibody
    • DMSMFH antibody
    • Epididymis luminal protein 249 antibody
    • HEL 249 antibody
    • LGMBF antibody
    • MeSAdo phosphorylase antibody
    • Methylthioadenosine phosphorylase antibody
    • MSAP antibody
    • MTA phosphorylase antibody
    • MTAP antibody
    • MTAP_HUMAN antibody
    • MTAPase antibody
    • S methyl 5 thioadenosine phosphorylase antibody
    • S methyl 5' thioadenosine phosphorylase antibody
    • S-methyl-5''-thioadenosine phosphorylase antibody
    see all

画像

  • Anti-MTAP antibody (ab96231) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 31 kDa
    12% SDS PAGE
  • ab96231, at a 1/500 dilution, staining Human MTAP in SAS xenograft, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

参考文献

ab96231 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA Buffer pH 8,5
Sample
Human Tissue sections (Pancreas)
Specification
Pancreas
Permeabilization
Yes - Wash-Buffer from Dako with Tween
Fixative
Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

投稿 Jul 14 2014

Thank you for your phone call.

I was unfortunately not able to find the rat sequence for MTAP (S-methyl-5'-thioadenosine phosphorylase) in both databases UniProt and NCBI. If you can find it, please send it to me for the sequence alignment. <...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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