製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MTA3
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within the C terminal amino acids 431-480 (TTEDPRVRSH VSRQAMQGMP VRNTGSPKSA VKTRQAFFLH TTYFTKFARQ) of Human MTA3 (NP_065795).

  • ポジティブ・コントロール
    • HeLa cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Plays a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels.
  • 組織特異性
    No expression in nonepithelial cells.
  • 配列類似性
    Contains 1 BAH domain.
    Contains 1 ELM2 domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 SANT domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1110002J22Rik antibody
    • fj99h01 antibody
    • KIAA1266 antibody
    • Metastasis associated 1 family, member 3 antibody
    • Metastasis associated 3 antibody
    • Metastasis associated family, member 3 antibody
    • Metastasis associated gene 3 antibody
    • Metastasis associated gene family, member 3 antibody
    • Metastasis associated protein MTA3 antibody
    • Metastasis-associated protein MTA3 antibody
    • MGC56396 antibody
    • MGC77410 antibody
    • mKIAA1266 antibody
    • mta1 antibody
    • Mta3 antibody
    • MTA3 metastasis associated 1 family, member 3 antibody
    • Mta3 metastasis associated 3 antibody
    • MTA3_HUMAN antibody
    • wu:fj99h01 antibody
    • zgc:56396 antibody
    see all

Anti-MTA3 antibody 画像

  • Anti-MTA3 antibody (ab82684) at 1 µg/ml (5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 59 kDa
    Observed band size : 59 kDa

プロトコール

Anti-MTA3 antibody (ab82684) 使用論文

ab82684 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for submitting this Abreview. As you might have seen, the results are now published on our Website. As the bands obtained with the antibody are rather faint, I would like to suggest to test another blocking buffer to improve the res...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - nuclear (brain and embryonic stem cell)
Loading amount
20 µg
Specification
brain and embryonic stem cell
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Erin Knock

Verified customer

投稿 Dec 09 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (embryonic day 18.5 brain)
Specification
embryonic day 18.5 brain
Fixative
Paraformaldehyde
Permeabilization
Yes - tween
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Erin Knock

Verified customer

投稿 Nov 21 2011

DISCOUNT CODE: *******Expiration date: ******** I am very pleased to hear you would like to accept our offer and test ab82684 in western blot on mouse tissue. This code will give you 1 free primary antibody before the expiration date. To redeem this of...

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DISCOUNT CODE: ****** Expiration date: ******* I am very pleased to hear you would like to accept our offer and test ab82684 in IHC on mouse tissue. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, pleas...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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