Anti-POLRMT 抗体 (ab228576)
Key features and details
- Rabbit polyclonal to POLRMT
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-POLRMT antibody
POLRMT 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to POLRMT -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment within Human POLRMT (internal sequence). The exact sequence is proprietary.
Database link: O00411 -
ポジティブ・コントロール
- ICC/IF: HepG2 cells. IHC-P: Human hepatoma tissue. WB: HepG2 whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab228576の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 139 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 139 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates. -
配列類似性
Belongs to the phage and mitochondrial RNA polymerase family. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 5442 Human
- Omim: 601778 Human
- SwissProt: O00411 Human
- Unigene: 254113 Human
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別名
- APOLMT antibody
- DNA-directed RNA polymerase antibody
- DNA-directed RNA polymerase mitochondrial antibody
see all
画像
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Anti-POLRMT antibody (ab228576) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 139 kDa5% SDS-PAGE
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Methanol-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells stained for POLRMT (green) using ab228576 (1/500 dilution) in ICC/IF.
Counterstain: Hoechst 33342 (blue).
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Paraffin-embedded human hepatoma tissue stained for POLRMT with ab228576 at 1/500 dilution in immunohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab228576 は 3 報の論文で使用されています。
- Wang Y et al. The mitochondrial RNA polymerase POLRMT promotes skin squamous cell carcinoma cell growth. Cell Death Discov 8:347 (2022). PubMed: 35922422
- Zhou T et al. The requirement of mitochondrial RNA polymerase for non-small cell lung cancer cell growth. Cell Death Dis 12:751 (2021). PubMed: 34326320
- Han QC et al. Identification of mitochondrial RNA polymerase as a potential therapeutic target of osteosarcoma. Cell Death Discov 7:393 (2021). PubMed: 34907167