製品の概要

  • 製品名Anti-MT-ND5 antibody
    MT-ND5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MT-ND5
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide between 550~579 amino acids selected from the C terminal region of Human MT-ND5 conjugated to KLH.

  • ポジティブ・コントロール
    • CEM cell line lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal cerebral cortex.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
IHC-P
  • 追加情報ELISA: 1/1000.
    WB: 1/100 - 1/500. Predicted molecular weight: 67 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
    • 関連疾患Defects in MT-ND5 are a cause of Leber hereditary optic neuropathy (LHON) [MIM:535000]. LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.
      Defects in MT-ND5 are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
      Defects in MT-ND5 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
      Defects in MT-ND5 are a cause of mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes syndrome (MELAS) [MIM:540000]. MELAS is a genetically heterogenious disorder, characterized by episodic vomiting, seizures, and recurrent cerebral insults resembling strokes and causing hemiparesis, hemianopsia, or cortical blindness.
    • 配列類似性Belongs to the complex I subunit 5 family.
    • 細胞内局在Mitochondrion inner membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Complex I, subunit ND5 antibody
      • EC 1.6.5.3 antibody
      • Mitochondrially encoded NADH dehydrogenase 5 antibody
      • MT ND5 antibody
      • MT-ND5 antibody
      • MTND 5 antibody
      • MTND5 antibody
      • NAD5 antibody
      • NADH dehydrogenase subunit 5 (complex I) antibody
      • NADH dehydrogenase subunit 5 antibody
      • NADH ubiquinone oxidoreductase , subunit ND5 antibody
      • NADH ubiquinone oxidoreductase chain 5 antibody
      • NADH-ubiquinone oxidoreductase chain 5 antibody
      • NADH5 antibody
      • ND5 antibody
      • NU5M_HUMAN antibody
      see all

    Anti-MT-ND5 antibody 画像

    • IHC image of MT-ND5 staining in Human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab92624, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

       

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Anti-MT-ND5 antibody (ab92624) at 1/1000 dilution + CEM whole cell lysate at 35 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

      Predicted band size : 67 kDa

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-MT-ND5 antibody (ab92624) at 1/100 dilution

      Lane 1 : Human Cerebral Cortex
      Lane 2 : Human Cerebral Cortex
      Lane 3 : Human Cerebral Cortex
      Lane 4 : Human Cerebral Cortex

      Lysates/proteins at 20 µg per lane.

      Secondary
      Donkey polyclonal to rabbit IgG conjugated to IRDye 800CW at 1/500 dilution

      Performed under reducing conditions.

      Predicted band size : 67 kDa


      Exposure time : 5 minutes

      Joseph Bateman, Kings College London, United Kingdom

      Detection method: Odyssey system

      See Abreview

    Anti-MT-ND5 antibody (ab92624) 使用論文

    This product has been referenced in:
    • Humphrey DM  et al. Alternative oxidase rescues mitochondria-mediated dopaminergic cell loss in Drosophila. Hum Mol Genet 21:2698-712 (2012). WB . Read more (PubMed: 22398207) »

    See 1 Publication for this product

    Product Wall

    Abreviews
    Application Western blot
    Sample Human Cell lysate - other (Mitochondrial fractions of HeLa WT and HeLa rho-0)
    Loading amount 26 µg
    Specification Mitochondrial fractions of HeLa WT and HeLa rho-0
    Gel Running Conditions Reduced Denaturing (4-15%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    投稿 Oct 26 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Tissue lysate - whole (Frontal cortex)
    Loading amount 20 µg
    Specification Frontal cortex
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Dr. Joseph Bateman

    Verified customer

    投稿 Oct 19 2012

    Thank you for contacting Abcam.

    We generally look for a minimum of 85% level of homology between an immunogen sequence and a target sequence before recommending an antibody.

    Since there is only a 65% level of homology between mouse an...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"