製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MSY2/YBOX2
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Guinea pig, Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (EPQKGGGAGG GGGAASGPAA GTPSAPGSRT PGNPATAVSG TPAPPARSQA) of human MSY2/YBOX2 (NP_057066).

  • ポジティブ・コントロール
    • Fetal lung lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:1562500.

ターゲット情報

Anti-MSY2/YBOX2 antibody 画像

  • Anti-MSY2/YBOX2 antibody (ab82963) at 1 µg/ml + fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 38 kDa
    Observed band size : 38 kDa

プロトコール

Anti-MSY2/YBOX2 antibody (ab82963) 使用論文

ab82963 has not yet been referenced specifically in any publications.

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Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Loading amount
30000 cells
Specification
293T
Gel Running Conditions
Reduced Denaturing (4-20% TrisGlycine)
Blocking step
5% Milk, 1% BSAS as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Feb 12 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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