製品の概要

  • 製品名Anti-Msx2/Hox8 antibody [2E12]
    Msx2/Hox8 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2E12] to Msx2/Hox8
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment:

    MASPSKGNDL FSPDEEGPAV VAGPGPGPGG AEGAAEERRV KVSSLPFSVE ALMSDKKPPK EASPLPAESA SAGATLR

    , corresponding to N terminal amino acids 1-77 of Human HOX8.

  • ポジティブ・コントロール
    • IHC-P: FFPE human ovary tissue sections.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab22601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 29 kDa).

ターゲット情報

  • 機能Acts as a transcriptional regulator in bone development. Represses the ALPL promoter activity and antogonizes the stimulatory effect of DLX5 on ALPL expression during osteoblast differentiation. Probable morphogenetic role. May play a role in limb-pattern formation. In osteoblasts, suppresses transcription driven by the osteocalcin FGF response element (OCFRE). Binds to the homeodomain-response element of the ALPL promoter.
  • 関連疾患Defects in MSX2 are the cause of parietal foramina 1 (PFM1) [MIM:168500]; also known as foramina parietalia permagna (FPP). PFM1 is an autosomal dominant disease characterized by oval defects of the parietal bones caused by deficient ossification around the parietal notch, which is normally obliterated during the fifth fetal month.
    Defects in MSX2 are the cause of parietal foramina with cleidocranial dysplasia (PFMCCD) [MIM:168550]; also known as cleidocranial dysplasia with parietal foramina. PFMCCD combines skull defects in the form of enlarged parietal foramina and deficient ossification of the clavicles.
    Defects in MSX2 are the cause of craniosynostosis type 2 (CRS2) [MIM:604757]; also known as craniosynostosis Boston-type (CSB). CRS2 is an autosomal dominant disorder characterized by the premature fusion of calvarial sutures. The craniosynostosis phenotype is either fronto-orbital recession, or frontal bossing, or turribrachycephaly, or cloverleaf skull. Associated features include severe headache, high incidence of visual problems (myopia or hyperopia), and short first metatarsals. Intelligence is normal.
  • 配列類似性Belongs to the Msh homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CRS 2 antibody
    • CRS2 antibody
    • FPP antibody
    • Homeo box msh like 2 antibody
    • Homeobox protein Hox-8 antibody
    • Homeobox protein MSX 2 antibody
    • Homeobox protein MSX-2 antibody
    • Homeobox protein MSX2 antibody
    • Hox 8 antibody
    • Hox8 antibody
    • MSH antibody
    • Msh homeo box 2 antibody
    • Msh homeo box homolog antibody
    • Msh homeo box homolog 2 antibody
    • Msh homeobox 2 antibody
    • Msh homeobox homolog 2 antibody
    • Msx 2 antibody
    • MSX2 antibody
    • MSX2_HUMAN antibody
    • Parietal foramina 1 antibody
    • PFM 1 antibody
    • PFM antibody
    • PFM1 antibody
    see all

Anti-Msx2/Hox8 antibody [2E12] 画像

  • IHC image of Msx2/Hox8 staining in human ovary formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22601, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Msx2/Hox8 antibody [2E12] (ab22601) 使用論文

This product has been referenced in:
  • Atsawasuwan P  et al. Ameloblastin inhibits cranial suture closure by modulating MSX2 expression and proliferation. PLoS One 8:e52800 (2013). WB ; Mouse . Read more (PubMed: 23593111) »

See 1 Publication for this product

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Thank you for your email. Below I have listed some suggestions for controls to use with these antibodies. If you have any additional questions, please contact Ab23798 - We do sell recombinant Il-1 beta, it is catalog numbers ab9617, ab9723, and ab97...

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