Anti-MST1/MST2 (phospho T180 + T183) 抗体 [EPR1467Y] (ab76323)

製品の概要

• 製品名
  Anti-MST1/MST2 (phospho T180 + T183) antibody [EPR1467Y]
  MST1/MST2 一次抗体 製品一覧
• 製品の詳細
  Rabbit monoclonal [EPR1467Y] to MST1/MST2 (phospho T180 + T183)
• アプリケーション
  適用あり: WBmore details
  適用なし: Flow Cyt,ICC,IHC-P or IP
• 種交差性
  交差種: Human
  
• 免疫原

  A phospho synthetic peptide corresponding to residues surrounding Threonine 183 of human MST1, or Threonine 180 of human MST2.

• ポジティブ･コントロール
  • HeLa cell lysates treated with Calyculin A.
• 特記事項

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

• 製品の状態
  Liquid
• 保存方法
  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• バッファー
  PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• 精製度
  Tissue culture supernatant
• ポリ/モノ
  モノクローナル
• クローン名
  EPR1467Y
• アイソタイプ
  IgG
• 研究分野
  • Cell Biology
  • Apoptosis
  • Intracellular
  • Kinases

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

アプリケーション

ターゲット情報

• 関連性
  The human serine/threonine kinase, mammalian STE20 like kinase (MST), is considerably homologous to the budding yeast kinases, SPS1 and STE20, throughout their kinase domains. When stably expressed in HeLa cells, MST highly sensitizes the cells to death receptor mediated apoptosis by accelerating caspase 3 activation. These findings suggest that MST1 and MST2 play a role in apoptosis both upstream and downstream of caspase activation. MST1 is cleaved and activated by caspases during apoptosis and is capable of inducing apoptotic morphological changes such as chromatin condensation upon overexpression. Mammalian Sterile 20 Like 2 (MST2) is most similar to the previously identified MST1 protein kinase (78% identity, 88% similarity). Northern analysis indicates that MST2 mRNA is expressed at high levels in adult kidney, skeletal and placental tissues and at very low levels in adult heart, lung, liver and brain tissues. An in vitro kinase assay indicates that MST2 can phosphorylate an exogenous substrate, as well as itself, and phospho amino acid analysis indicates that it is a serine/threonine protein kinase.
• 細胞内局在
  Cytoplasm. Nucleus. Note: The caspase cleaved form cycles between the nucleus and cytoplasm.
• 参照データベース
  • Entrez Gene: 6788 Human
  • Entrez Gene: 6789 Human
  • Omim: 604965 Human
  • Omim: 605030 Human
  • SwissProt: Q13043 Human
  • SwissProt: Q13188 Human
• 別名
  • DKFZp686A2068 antibody
  • FLJ90748 antibody
  • KRS1 antibody

  • KRS2 antibody
  • Mammalian STE20 like protein kinase 1 antibody
  • Mammalian STE20 like protein kinase 2 antibody
  • MST 1 antibody
  • MST 2 antibody
  • MST1 antibody
  • MST2 antibody
  • Serine/threonine kinase 3 (STE20 homolog yeast) antibody
  • Serine/threonine kinase 3 (Ste20 yeast homolog) antibody
  • Serine/threonine kinase 3 antibody
  • Serine/threonine kinase 4 antibody
  • Serine/threonine protein kinase 3 antibody
  • Serine/threonine protein kinase 4 antibody
  • Serine/threonine protein kinase Krs 1 antibody
  • Serine/threonine protein kinase Krs 2 antibody
  • STE20 like kinase MST1 antibody
  • STE20 like kinase MST2 antibody
  • STK 3 antibody
  • STK 4 antibody
  • STK3 antibody
  • STK4 antibody
  • YSK3 antibody

  see all

プロトコール

• Western blot protocols