製品の概要

  • 製品名Anti-MSK2 / RSK-B antibody
    MSK2 / RSK-B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MSK2 / RSK-B
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide, corresponding to a region between residues 722 - 772 of Human MSK2/ RSK-B (NP_003933.1).

  • ポジティブ・コントロール
    • HeLa, 293T or NIH 3T3 cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab99411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 86 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. Essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF) and result in the transcriptional activation of several immediate early genes, including proto-oncogenes FOS and JUN (By similarity). Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).
  • 配列類似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 2 protein kinase domains.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 90 kDa ribosomal protein S6 kinase 4 antibody
    • EC 2.7.11.1 antibody
    • KS6A4_HUMAN antibody
    • Mitogen and stress activated protein kinase 2 antibody
    • Nuclear mitogen and stress activated protein kinase 2 antibody
    • Nuclear mitogen- and stress-activated protein kinase 2 antibody
    • Ribosomal protein kinase B antibody
    • Ribosomal protein S6 kinase 90kD polypeptide 4 antibody
    • Ribosomal protein S6 kinase 90kDa polypeptide 4 antibody
    • Ribosomal protein S6 kinase alpha 4 antibody
    • Ribosomal protein S6 kinase alpha-4 antibody
    • RPS6KA4 antibody
    • RSKB antibody
    • S6K-alpha-4 antibody
    see all

Anti-MSK2 / RSK-B antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MSK2 / RSK-B with ab99411 at 1/200 (1µg/mg). Detection: DAB.
  • All lanes : Anti-MSK2 / RSK-B antibody (ab99411) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH 3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 86 kDa


    Exposure time : 30 seconds
  • Detection of MSK2 / RSK-B in immunoprecipitates of HeLa whole cell lysate (1 mg lysate for IP, 20% of IP loaded on gel) using ab99411 at 3 µg/mg lysate for IP, and at 1 µg/ml for the subsequent western blot.

    Detection: chemiluminescence with an exposure time of 30 seconds.

Anti-MSK2 / RSK-B antibody (ab99411) 使用論文

ab99411 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"