Anti-MSK1 (phospho S376) 抗体 (ab51041)


  • 製品名
    Anti-MSK1 (phospho S376) antibody
    MSK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MSK1 (phospho S376)
  • 特異性
    The antibody detects endogenous levels of MSK1 only when phosphorylated at serine 376
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human MSK1 aa 343-392.

  • ポジティブ・コントロール
    • HeLa cells treated with PMA



Our Abpromise guarantee covers the use of ab51041 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 86 kDa (predicted molecular weight: 90 kDa).
ELISA 1/20000.


  • 機能
    Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).
  • 組織特異性
    Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.
  • 配列類似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 2 protein kinase domains.
  • 翻訳後修飾
    Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain.
  • 細胞内局在
    Nucleus. Cytoplasm. Predominantly nuclear. Partially cytoplasmic.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 90 kDa ribosomal protein S6 kinase 5 antibody
    • EC antibody
    • KS6A5_HUMAN antibody
    • MGC1911 antibody
    • Mitogen and stress activated protein kinase 1 antibody
    • MSPK1 antibody
    • Nuclear Mitogen And Stress Activated Protein Kinase 1 antibody
    • Nuclear mitogen- and stress-activated protein kinase 1 antibody
    • Ribosomal protein S6 kinase 90kD polypeptide 5 antibody
    • Ribosomal protein S6 kinase 90kDa antibody
    • Ribosomal protein S6 kinase 90kDa polypeptide 5 antibody
    • Ribosomal Protein S6 Kinase Alpha 5 antibody
    • Ribosomal protein S6 kinase alpha-5 antibody
    • RLPK antibody
    • RPS6KA5 antibody
    • RSK Like Protein Kinase antibody
    • RSK-like protein kinase antibody
    • RSKL antibody
    • S6K alpha 5 antibody
    • S6K-alpha-5 antibody
    see all


  • All lanes : Anti-MSK1 (phospho S376) antibody (ab51041) at 1/500 dilution

    Lane 1 : Hela whole cell lysate, cells treated with PMA (125ng/ml for 15minutes)
    Lane 2 : Hela whole cell lysate

    Predicted band size : 90 kDa



ab51041 has not yet been referenced specifically in any publications.

レビューと Q&A

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Indeed, I can confirm that we received and published the abreview, thus the discount code x is now active and can be used to geta free primary antibody.

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Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Mouse Cell lysate - nuclear (pituitary)
Loading amount
10 µg
GnRH 20 min to 120 min
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

投稿 Mar 30 2012

Thank you very much for contacting us. Of course, she could test the antibody in ChIP instead (or additionally, if she likes). As ChIP has not been tested until now though, we would recommend, testing it with human samples first, as we can guarantee...

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DISCOUNT CODE: xxx Expiration date: dd mm 2012 I am very pleased to hear she would like to accept our offer and test ab51041 in mouse. This code will give 1 free primary antibody before the expiration date. To redeem this offer, please submit an Ab...

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