製品の概要

  • 製品名
    Anti-MSK1 (phospho S360) antibody [EP1888Y]
    MSK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1888Y] to MSK1 (phospho S360)
  • 特異性
    ab81294 will detect MSK1 phosphorylated on Serine 360.
  • アプリケーション
    適用あり: IP, WB, ICC/IFmore details
    適用なし: Flow Cyt or IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic phospho peptide corresponding to residues surrounding Serine 360 of human MSK1

  • ポジティブ・コントロール
    • HEK293 cell lyasate.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab81294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP 1/30.
WB 1/5000 - 1/20000. Predicted molecular weight: 90 kDa.
ICC/IF 1/100 - 1/250.
  • 追加情報
    Is unsuitable for Flow Cyt or IHC-P.
  • ターゲット情報

    • 機能
      Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).
    • 組織特異性
      Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.
    • 配列類似性
      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 2 protein kinase domains.
    • 翻訳後修飾
      Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain.
    • 細胞内局在
      Nucleus. Cytoplasm. Predominantly nuclear. Partially cytoplasmic.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 90 kDa ribosomal protein S6 kinase 5 antibody
      • EC 2.7.11.1 antibody
      • KS6A5_HUMAN antibody
      • MGC1911 antibody
      • Mitogen and stress activated protein kinase 1 antibody
      • MSPK1 antibody
      • Nuclear Mitogen And Stress Activated Protein Kinase 1 antibody
      • Nuclear mitogen- and stress-activated protein kinase 1 antibody
      • Ribosomal protein S6 kinase 90kD polypeptide 5 antibody
      • Ribosomal protein S6 kinase 90kDa antibody
      • Ribosomal protein S6 kinase 90kDa polypeptide 5 antibody
      • Ribosomal Protein S6 Kinase Alpha 5 antibody
      • Ribosomal protein S6 kinase alpha-5 antibody
      • RLPK antibody
      • RPS6KA5 antibody
      • RSK Like Protein Kinase antibody
      • RSK-like protein kinase antibody
      • RSKL antibody
      • S6K alpha 5 antibody
      • S6K-alpha-5 antibody
      see all

    画像

    • All lanes : Anti-MSK1 (phospho S360) antibody [EP1888Y] (ab81294) at 1/20000 dilution

      Lane 1 : HEK293 cell lyasate, untreated,
      Lane 2 : HEK293 cell lyasate treated with AP.

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti rabbit. at 1/2000 dilution

      Predicted band size : 90 kDa
      Observed band size : 90 kDa
    • ICC/IF image of ab81294 stained MCF7 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81294, neat) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    参考文献

    This product has been referenced in:
    • Wiersma M  et al. Protein kinase Msk1 physically and functionally interacts with the KMT2A/MLL1 methyltransferase complex and contributes to the regulation of multiple target genes. Epigenetics Chromatin 9:52 (2016). WB ; Human . Read more (PubMed: 27895715) »
    • Ufelmann H & Schrenk D Nodularin-triggered apoptosis and hyperphosphorylation of signaling proteins in cultured rat hepatocytes. Toxicol In Vitro 29:16-26 (2015). Read more (PubMed: 25192816) »

    See all 5 Publications for this product

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