Anti-MSK1 (phospho S212) 抗体 (ab79499)

製品の概要

• 製品名
  Anti-MSK1 (phospho S212) antibody
  MSK1 一次抗体 製品一覧
• 製品の詳細
  Rabbit polyclonal to MSK1 (phospho S212)
• 特異性
  ab79499 detects endogenous levels of MSK1 only when phosphorylated at serine 212
• アプリケーション
  適用あり: WB, IHC-P, ELISAmore details
• 種交差性
  交差種: Human
  交差が予測される動物種: Mouse
• 免疫原

  Synthetic phosphopeptide derived from human MSK1 around the phosphorylation site of serine 212 (AYS^PFC)

• ポジティブ･コントロール
  • IHC: human breast carcinoma tissue WB: extracts from 3T3 cells (treated with EGF at 200 ng/ml for 5mins)

製品の特性

• 製品の状態
  Liquid
• 保存方法
  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• バッファー
  Preservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• 精製度
  Immunogen affinity purified
• 特記事項（精製）
  Purified by affinity chromatography using an epitope specific phosphopeptide. The antibody against the non-phosphopeptide was removed by chromatography using a non-phosphopeptide corresponding to the phosphorylation site.
• ポリ/モノ
  ポリクローナル
• アイソタイプ
  IgG
• 研究分野
  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Receptor Tyrosine Kinases

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

  • Cell Biology
  • Other Antibodies
  • Oxidative Stress

  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Oxidative stress

関連製品

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

アプリケーション

ターゲット情報

• 機能
  Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).
• 組織特異性
  Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.
• 配列類似性
  Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
  Contains 1 AGC-kinase C-terminal domain.
  Contains 2 protein kinase domains.
• 翻訳後修飾
  Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain.
• 細胞内局在
  Nucleus. Cytoplasm. Predominantly nuclear. Partially cytoplasmic.

• Target information above from: UniProt accession O75582 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 参照データベース
  • Entrez Gene: 9252 Human
  • Entrez Gene: 73086 Mouse
  • Omim: 603607 Human
  • SwissProt: O75582 Human
  • SwissProt: Q8C050 Mouse
  • Unigene: 510225 Human
  • Unigene: 220417 Mouse
  • Unigene: 392855 Mouse
• 別名
  • 90 kDa ribosomal protein S6 kinase 5 antibody
  • EC 2.7.11.1 antibody
  • KS6A5_HUMAN antibody

  • MGC1911 antibody
  • Mitogen and stress activated protein kinase 1 antibody
  • MSPK1 antibody
  • Nuclear Mitogen And Stress Activated Protein Kinase 1 antibody
  • Nuclear mitogen- and stress-activated protein kinase 1 antibody
  • Ribosomal protein S6 kinase 90kD polypeptide 5 antibody
  • Ribosomal protein S6 kinase 90kDa antibody
  • Ribosomal protein S6 kinase 90kDa polypeptide 5 antibody
  • Ribosomal Protein S6 Kinase Alpha 5 antibody
  • Ribosomal protein S6 kinase alpha-5 antibody
  • RLPK antibody
  • RPS6KA5 antibody
  • RSK Like Protein Kinase antibody
  • RSK-like protein kinase antibody
  • RSKL antibody
  • S6K alpha 5 antibody
  • S6K-alpha-5 antibody

  see all

プロトコール

• Western blot protocols
• Immunohistochemistry protocols