製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MSI2
  • アプリケーション
    適用あり: ELISA, IHC-Fr, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human, Apteronotus leptorhynchus
    交差が予測される動物種: Rat, Chicken, Cow, Dog, Pig, Caenorhabditis elegans, Chimpanzee, Drosophila melanogaster, Zebrafish
  • 免疫原

    A region within synthetic peptide: LRDYFSKFGE IRECMVMRDP TTKRSRGFGF VTFADPASVD KVLGQPHHEL, corresponding to N terminal amino acids 37-86 of Human MSI2

  • ポジティブ・コントロール
    • HepG2 cell lysate, human liver tissue and human heart tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 機能
    RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
  • 組織特異性
    Ubiquitous; detected at low levels.
  • 関連疾患
    Note=Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23).
  • 配列類似性
    Belongs to the Musashi family.
    Contains 2 RRM (RNA recognition motif) domains.
  • 翻訳後修飾
    Phosphorylated.
  • 細胞内局在
    Cytoplasm. Associated with polysomes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ36569 antibody
    • MGC3245 antibody
    • Msi2 antibody
    • MSI2/HOXA9 fusion gene, included antibody
    • MSI2H antibody
    • MSI2H_HUMAN antibody
    • Musashi 2 antibody
    • Musashi homolog 2 antibody
    • Musashi RNA binding protein 2 antibody
    • Musashi, Drosophila, homolog of, 2 antibody
    • Musashi-2 antibody
    • RNA binding protein Musashi homolog 2 antibody
    • RNA-binding protein Musashi homolog 2 antibody
    • WD 40 repeat protein MSI2 antibody
    see all

画像

  • All lanes : Anti-MSI2 antibody (ab50829) at 2.5

    Lane 1 : HepG2 cell lysate
    Lane 2 : HepG2 cell lysate with blocking peptide at 2 µg/ml

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 35 kDa
  • Ab50829 (8ug/ml) staining human MSI2 in human liver tissue by immunohistochemistry using paraffin embedded tissue. Hepatocytes (indicated with arrow) were positively stained.
  • AB50829 (8ug/ml) staining human MSI2 in human heart tissue by immunohistochemistry using paraffin embedded tissue. Myocardial cells (indicated with arrows) were positively stained.
  • ab50829 staining MSI2 in 16 µm thick transverse sections of adult brown ghost knifefish spinal cord by Immunohistochemistry (Frozen sections).
    Tissue was fixed in paraformaldehyde, permeabilized using 0.3% Triton X-100, blocked with 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS for 1 hour at room temperature and then incubated with ab50829 at a 1/25 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/200 dilution.

    See Abreview

  • Anti-MSI2 antibody (ab50829) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG (1:100,000)

    Predicted band size : 35 kDa
    Observed band size : 35 kDa

参考文献

This product has been referenced in:
  • Sutherland JM  et al. Knockout of RNA Binding Protein MSI2 Impairs Follicle Development in the Mouse Ovary: Characterization of MSI1 and MSI2 during Folliculogenesis. Biomolecules 5:1228-44 (2015). WB ; Mouse . Read more (PubMed: 26131972) »
  • Sutherland JM  et al. RNA binding protein Musashi-1 directly targets Msi2 and Erh during early testis germ cell development and interacts with IPO5 upon translocation to the nucleus. FASEB J 29:2759-68 (2015). Read more (PubMed: 25782991) »

See all 3 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Jan 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Spinal cord)
Specification
Spinal cord
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Dr. Ruxandra Sirbulescu

Verified customer

投稿 Dec 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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