製品の概要

  • 製品名Anti-MSI2 antibody
    MSI2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MSI2
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse MSI2.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in U87MG whole cell lysate as well as both Mouse Brain Mouse Spinal cord tissue lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab105889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).

ターゲット情報

  • 機能RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
  • 組織特異性Ubiquitous; detected at low levels.
  • 関連疾患Note=Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23).
  • 配列類似性Belongs to the Musashi family.
    Contains 2 RRM (RNA recognition motif) domains.
  • 翻訳後修飾Phosphorylated.
  • 細胞内局在Cytoplasm. Associated with polysomes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ36569 antibody
    • MGC3245 antibody
    • Msi2 antibody
    • MSI2/HOXA9 fusion gene, included antibody
    • MSI2H antibody
    • MSI2H_HUMAN antibody
    • Musashi 2 antibody
    • Musashi homolog 2 antibody
    • Musashi RNA binding protein 2 antibody
    • Musashi, Drosophila, homolog of, 2 antibody
    • Musashi-2 antibody
    • RNA binding protein Musashi homolog 2 antibody
    • RNA-binding protein Musashi homolog 2 antibody
    • WD 40 repeat protein MSI2 antibody
    see all

Anti-MSI2 antibody 画像

  • All lanes : Anti-MSI2 antibody (ab105889) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Spinal Cord (Mouse) Tissue Lysate
    Lane 3 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 37 kDa
    Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab105889 stained Mouse Embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab105889 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-MSI2 antibody (ab105889) 使用論文

ab105889 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"