製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MSH4
  • アプリケーション
    適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    A synthetic peptide corresponding to C terminal residues of Mouse MSH4

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab58666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 19247432
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 105 kDa.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Involved in meiotic recombination. Required for reciprocal recombination and proper segregation of homologous chromosomes at meiosis.
  • 組織特異性
    Testis and ovary specific.
  • 配列類似性
    Belongs to the DNA mismatch repair MutS family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hMSH4 antibody
    • MSH 4 antibody
    • MSH4 antibody
    • MSH4_HUMAN antibody
    • MutS homolog 4 (E. Coli) antibody
    • MutS homolog 4 antibody
    • MutS protein homolog 4 antibody
    see all

画像

  • ab58666 staining MSH4 in mouse spermatocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed in 1% paraformaldehyde and 0.15% Triton X-100. An Alexa flour conjugated anti rabbit IgG was used as secondary. Green colour staining was observed with ab58666.

参考文献

This product has been referenced in:
  • Rong M  et al. Meiotic cohesin subunits RAD21L and REC8 are positioned at distinct regions between lateral elements and transverse filaments in the synaptonemal complex of mouse spermatocytes. J Reprod Dev 62:623-630 (2016). IF ; Mouse . Read more (PubMed: 27665783) »
  • Qiao H  et al. Antagonistic roles of ubiquitin ligase HEI10 and SUMO ligase RNF212 regulate meiotic recombination. Nat Genet 46:194-9 (2014). IF . Read more (PubMed: 24390283) »

See all 11 Publications for this product

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (spermatocytes)
Permeabilization
No
Specification
spermatocytes
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 27°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 01 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録