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Ni-NTA purified recombinant human MSH2 expressed in E. Coli strain BL21 (DE3).
Our Abpromise guarantee covers the use of ab52266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|ICC/IF||1/200 - 1/1000.|
|IHC-Fr||1/200 - 1/1000.|
|WB||1/500 - 1/2000. Detects a band of approximately 98 kDa (predicted molecular weight: 105 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|IP||Use a concentration of 5 µg/ml.|
ab52266 at 1/1000 dilution staining MSH2 in human Hela cells by Immunocytochemistry/ Immunofluorescence. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG1 was used as secondary antibody. The primary antibody shows green staining in image whilst actin filaments were stained red with Alexa Fluor® 555 phalloidin.