製品の概要

  • 製品名Anti-MSF antibody
    MSF 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MSF
  • アプリケーション適用あり: IP, IHC-Pmore details
    適用なし: WB
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rhesus monkey
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 536 - 586 of Human MSF (NP_001106963.1).

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH: 7 to 8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab114099 was affinity purified using an epitope specific to MSF immobilized on solid support.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab114099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 機能Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.
    • 組織特異性Widely expressed. Isoforms are differentially expressed in testes, kidney, liver heart, spleen, brain, peripheral blood leukocytes, skeletal muscle and kidney. Specific isoforms appear to demonstrate tissue specificity. Isoform 5 is the most highly expressed in fetal tissue. Isoform 1 is detected in all tissues except the brain and thymus, while isoform 2, isoform 3, and isoform 4 are detected at low levels in approximately half of the fetal tissues.
    • 関連疾患Note=A chromosomal aberration involving SEPT9/MSF is found in therapy-related acute myeloid leukemia (t-AML). Translocation t(11;17)(q23;q25) with MLL.
      Defects in SEPT9 are a cause of hereditary neuralgic amyotrophy (HNA) [MIM:162100]; also known as neuritis with brachial predilection (NAPB) or hereditary brachial plexus neuropathy or hereditary neuralgic amyotrophy with predilection for brachial plexus. HNA is an autosomal dominant form of recurrent focal neuropathy characterized clinically by acute, recurrent episodes of brachial plexus neuropathy with muscle weakness and atrophy preceded by severe pain in the affected arm. HNA is triggered by environmental factors such as infection or parturition.
    • 配列類似性Belongs to the septin family.
    • 細胞内局在Cytoplasm > cytoskeleton. In an epithelial cell line, concentrates at cell-cell contact areas. After TGF-beta1 treatment and induction of epithelial to mesenchymal transition, colocalizes partly with actin stress fibers. During bacterial infection, displays a collar shape structure next to actin at the pole of invading bacteria.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AF17q25 antibody
      • Cell division control protein septin D1 antibody
      • KIAA0991 antibody
      • MLL septin like fusion protein antibody
      • MLL septin-like fusion protein antibody
      • MLL septin-like fusion protein MSF-A antibody
      • MSF antibody
      • MSF1 antibody
      • NAPB antibody
      • Ov/Br septin antibody
      • Ovarian/breast septin alpha antibody
      • Ovarian/Breast septin antibody
      • PNUTL4 antibody
      • SEPT9 antibody
      • SEPT9_HUMAN antibody
      • SeptD1 antibody
      • Septin 9 antibody
      • Septin D1 antibody
      • Septin-9 antibody
      • SINT1 antibody
      see all

    Anti-MSF antibody 画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MSF with ab114099 at 1/1000 (1µg/ml). A DyLight® 594-conjugated goat anti-rabbit IgG (H+L) (1/100) was used as the secondary antibody.
    • Rabbit anti-MSF antibody, at 1 µg/ml, staining MSF in HeLa whole cell lysate immunoprecipitated using ab114099 at 3 µg/mg lysate (1 mg for IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 1 second.

    Anti-MSF antibody (ab114099) 使用論文

    ab114099 has not yet been referenced specifically in any publications.

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