製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MRPS15
  • アプリケーション
    適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: RGYVVRKPAQ SRLDDDPPPS TLLKDYQNVP GIEKVDDVVK RLLSLEMANK , corresponding to amino acids 51-100 of Human MRPS15

  • ポジティブ・コントロール
    • 721 B Lymphoblast cell lysate and human lung tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab69869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/12500.

WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling MRPS15 with ab69869 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining seen in the plasma membrane. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - MRPS15. Right - Merge.
  • Anti-MRPS15 antibody (ab69869) at 0.5 µg/ml + 721 B Lymphoblast cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 50000

    Predicted band size : 30 kDa
    Observed band size : 30 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling MRPS15 with ab69869 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.

参考文献

This product has been referenced in:
  • Davies SM  et al. Pentatricopeptide repeat domain protein 3 associates with the mitochondrial small ribosomal subunit and regulates translation. FEBS Lett 583:1853-8 (2009). WB ; Human . Read more (PubMed: 19427859) »

See 1 Publication for this product

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