Anti-MRPL41 抗体 (ab121821)
Key features and details
- Rabbit polyclonal to MRPL41
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MRPL41 antibody -
製品の詳細
Rabbit polyclonal to MRPL41 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
antigen sequence: KPYVSYLAPE SEETPLTAAQ LFSEAVAPAI EKDFKDGTFD PDNLEKYGFE PTQEGKLFQL YPRNFLR, corresponding to amino acids 71-137 of Human MRPL41.
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ポジティブ・コントロール
- Human rectum tissue; RT-4 and U-251 MG cell lysates; U-251MG cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121821の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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特記事項 |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
ターゲット情報
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機能
Component of the mitochondrial ribosome large subunit. Also involved in apoptosis and cell cycle. Enhances p53/TP53 stability, thereby contributing to p53/TP53-induced apoptosis in response to growth-inhibitory condition. Enhances p53/TP53 translocation to the mitochondria. Has the ability to arrest the cell cycle at the G1 phase, possibly by stabilizing the CDKN1A and CDKN1B (p27Kip1) proteins. -
組織特異性
Present in kidney, liver, thymus and testis, and at lower level in brain and spleen (at protein level). -
配列類似性
Belongs to the ribosomal protein L41 family. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 64975 Human
- Omim: 611846 Human
- SwissProt: Q8IXM3 Human
- Unigene: 44017 Human
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別名
- 39S ribosomal protein L27 homolog antibody
- 39S ribosomal protein L41 antibody
- 39S ribosomal protein L41 mitochondrial antibody
see all
画像
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ab121821, at 1/300 dilution, staining MRPL41 in paraffin-embedded Human rectum tissue by Immunohistochemistry.
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All lanes : Anti-MRPL41 antibody (ab121821) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate -
ab121821, at 4µg/ml, staining MRPL41 in U-251MG cells by Immunofluorescence. Antibody staining is shown in green.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab121821 は 1 報の論文で使用されています。
- Moreno-Asso A et al. Non-cell autonomous mechanisms control mitochondrial gene dysregulation in polycystic ovary syndrome. J Mol Endocrinol 68:63-76 (2021). PubMed: 34752415