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Our Abpromise guarantee covers the use of ab39268 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|ELISA||Use at an assay dependent concentration. PubMed: 20144953
This antibody gave a positive result in ELISA against the immunizing peptide. Antibody detection limit dilution >1:32,000.
|WB||Use at an assay dependent concentration.|
ab39268 stained Caco-2 cells. The cells were 100% methamol fixed for 5 minutes at room temperature and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39268 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was 150133 used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.