製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to MRPL3
  • アプリケーション
    適用あり: ICC/IF, ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    NVCQPGAPSITFA

    , corresponding to C terminal amino acids 336-348 of Human MRPL3

  • ポジティブ・コントロール
    • This antibody gave a positive result when used in the following methanol fixed cell lines: Caco-2

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab39268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration. PubMed: 20144953

This antibody gave a positive result in ELISA against the immunizing peptide. Antibody detection limit dilution >1:32,000.

WB Use at an assay dependent concentration.

ターゲット情報

  • 配列類似性
    Belongs to the ribosomal protein L3P family.
  • 細胞内局在
    Mitochondrion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 39S ribosomal protein L3 antibody
    • 39S ribosomal protein L3 mitochondrial antibody
    • COXPD9 antibody
    • L3mt antibody
    • Mitochondrial 39S ribosomal protein L3 antibody
    • Mitochondrial 60S ribosomal protein L3 antibody
    • mitochondrial antibody
    • mitochondrial ribosomal protein antibody
    • Mitochondrial ribosomal protein L3 antibody
    • MRL3 antibody
    • MRP-L3 antibody
    • MRPL3 antibody
    • RM03_HUMAN antibody
    • RPML3 antibody
    see all

画像

  • ab39268 stained Caco-2 cells. The cells were 100% methamol fixed for 5 minutes at room temperature and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39268 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was 150133 used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

参考文献

This product has been referenced in:
  • Zaganelli S  et al. The Pseudouridine Synthase RPUSD4 Is an Essential Component of Mitochondrial RNA Granules. J Biol Chem 292:4519-4532 (2017). Read more (PubMed: 28082677) »
  • Rozanska A  et al. The human RNA-binding protein RBFA promotes the maturation of the mitochondrial ribosome. Biochem J 474:2145-2158 (2017). WB . Read more (PubMed: 28512204) »

See all 10 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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