製品の概要

  • 製品名Anti-MRP8 + MRP14 antibody [MAC 387]
    MRP8 + MRP14 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MAC 387] to MRP8 + MRP14
  • 特異性This antibody has been shown as an important marker for identifying macrophages in tissue sections. It reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia.
  • アプリケーション適用あり: Flow Cyt, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Guinea pig, Cat, Dog, Human, Pig, Monkey, Baboon
  • 免疫原

    Affinity purified monocyte membrane preparation.

  • ポジティブ・コントロール
    • Tonsil, lymph node or spleen.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab80708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P 1/1000. We recommend using a TRIS/EDTA buffer pH 9.0.

ターゲット情報

  • 関連性MRP8 and MRP14 are members of the S100 family of proteins containing 2 EF hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21.
  • 細胞内局在Cytoplasmic and Nuclear
  • 参照データベース
  • 別名
    • CAGA antibody
    • CAGB antibody
    • Calgranulin A antibody
    • Calgranulin B antibody
    • Calprotectin L1L subunit antibody
    • CFAG antibody
    • CP10 antibody
    • Cystic fibrosis antigen antibody
    • Leukocyte L1 complex light chain antibody
    • Macrophage L1 protein Calprotectin antibody
    • Migration inhibitory factor related protein 14 antibody
    • Migration inhibitory factor related protein 8 antibody
    • MRP-14 antibody
    • MRP-8 antibody
    • P14 antibody
    • P8 antibody
    • S100 calcium binding protein A8 antibody
    • S100 calcium binding protein A9 antibody
    • S100A8 antibody
    • S100A9 antibody
    • Urinary stone protein band A antibody
    see all

Anti-MRP8 + MRP14 antibody [MAC 387] 画像

  • Formalin-fixed, paraffin-embedded human tonsil stained with ab80708 at a 1/1000 dilution using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of macrophages.
  • Overlay histogram showing THP1 cells stained with ab80708 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab80708, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-MRP8 + MRP14 antibody [MAC 387] (ab80708) 使用論文

This product has been referenced in:
  • Andersen CB  et al. Acute kidney graft rejection. A morphological and immunohistological study on "zero-hour" and follow-up biopsies with special emphasis on cellular infiltrates and adhesion molecules. APMIS 102:23-37 (1994). IHC-P ; Human, Mouse, Rat, Baboon, Cat, Dog, Guinea pig, Monkey, Pig . Read more (PubMed: 7513171) »
  • Coleman N & Stanley MA Expression of the myelomonocytic antigens CD36 and L1 by keratinocytes in squamous intraepithelial lesions of the cervix. Hum Pathol 25:73-9 (1994). IHC-P ; Human, Mouse, Rat, Baboon, Cat, Dog, Guinea pig, Monkey, Pig . Read more (PubMed: 7508885) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (kidney)
Antigen retrieval step None
Permeabilization No
Specification kidney
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative Paraformaldehyde
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投稿 Feb 12 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"