Recombinant MRP8/14 heterocomplex (Human). The antigen is produced by the Ca2+ dependent heterocomplex formation of MRP8 (S100A8 or Calgranulin A) and MRP14 (S100A9 or Calgranulin B), two calcium binding proteins of the S 100 protein family.
特記事項Peripheral blood monocytes carry the antigen extra and intracellularly, neutrophils only intracellularly. The antigen is present on macrophages in the red pulp of the spleen and in the liver. It is also strongly expressed on macrophages in acute inflamed tissues (peridontitis, contact excema, urticaria, erythrodermia).
In immune histochemistry, this antibody detects early inflammatory macrophages and can thus be used for the classification of acute stage inflammation in tissue sections and in smears.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 µg/ml.
Use a concentration of 4 µg/ml.
1/250. Proteinase K pretreatment for antigen retrieval is recommended.
関連性MRP8 and MRP14 are members of the S100 family of proteins containing 2 EF hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21.
Lood C et al. Protein synthesis of the pro-inflammatory S100A8/A9 complex in plasmacytoid dendritic cells and cell surface S100A8/A9 on leukocyte subpopulations in systemic lupus erythematosus. Arthritis Res Ther13:R60 (2011).
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