製品の概要

  • 製品名Anti-MRP8 antibody [EPR3554]
    MRP8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3554] to MRP8
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
    適用なし: Flow Cyt or ICC/IF
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MRP8 aa 50 to the C-terminus (C terminal).
    Database link: P05109

  • ポジティブ・コントロール
    • WB: Human (adult and fetal) and mouse spleen tissue lysates and PMBC and HL-60 cell lysates. IHC-P: Human cervical carcinoma, breast carcinoma, tonsil and spleen tissues. IP: HL-60 cell lysate.
  • 特記事項

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • クローン名EPR3554
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab92331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 11 kDa.

For unpurified use at 1/2000 - 1/10000.

IP 1/30.

For unpurified use at 1/20.

IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

For unpurified use at 1/100 - 1/250.

  • 追加情報Is unsuitable for Flow Cyt or ICC/IF.
  • ターゲット情報

    • 機能Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
    • 組織特異性Expressed by macrophages in chronic inflammations. Also expressed in epithelial cells constitutively or induced during dermatoses.
    • 配列類似性Belongs to the S-100 family.
      Contains 2 EF-hand domains.
    • 細胞内局在Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 60B8Ag antibody
      • AI323541 antibody
      • B8Ag antibody
      • BEE11 antibody
      • CAGA antibody
      • Calgranulin-A antibody
      • Calprotectin L1L subunit antibody
      • Calprotectin, included antibody
      • CFAG antibody
      • CGLA antibody
      • Chemotactic cytokine CP-10 antibody
      • CP-10 antibody
      • Cystic fibrosis antigen antibody
      • L1Ag antibody
      • Leukocyte L1 complex light chain antibody
      • MA387 antibody
      • MIF antibody
      • Migration inhibitory factor-related protein 8 antibody
      • MRP-8 antibody
      • Myeloid-related protein 8 antibody
      • Neutrophil cytosolic 7 kDa protein antibody
      • NIF antibody
      • p8 antibody
      • Pro-inflammatory S100 cytokine antibody
      • Protein S100-A8 antibody
      • S100 calcium binding protein A8 (calgranulin A) antibody
      • S100 calcium binding protein A8 antibody
      • S100 calcium-binding protein A8 antibody
      • S100A8 antibody
      • S100A8/S100A9 complex, included antibody
      • S10A8_HUMAN antibody
      • Urinary stone protein band A antibody
      see all

    Anti-MRP8 antibody [EPR3554] 画像

    • All lanes : Anti-MRP8 antibody [EPR3554] (ab92331) at 1/20000 dilution (purified)

      Lane 1 : HL-60 cell lysate
      Lane 2 : Human fetal spleen tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 11 kDa
      Observed band size : 11 kDa

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-MRP8 antibody [EPR3554] (ab92331) at 1/2000 dilution (purified) + Mouse spleen tissue lysate at 20 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 11 kDa
      Observed band size : 11 kDa

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-MRP8 antibody [EPR3554] (ab92331) (unpurified)

      Lane 1 : Human spleen tissue lysate
      Lane 2 : PMBC cell lysate
      Lane 3 : HL-60 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 11 kDa
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labelling MRP8 with purified ab92331 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labelling MRP8 with unpurified ab92331. Detection: DAB staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MRP8 with unpurified ab92331.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling MRP8 with unpurified ab92331. Detection: DAB staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling MRP8 with unpurified ab92331.

    • ab92331 (purified) at 1/30 immunoprecipitating MRP8 in HL-60 cell lysate (Lane 1). Lane 2 - rabbit monoclonal IgG instead of ab92331 in HL-60 lysates. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    Anti-MRP8 antibody [EPR3554] (ab92331) 使用論文

    This product has been referenced in:
    • Wang Y  et al. Platelet-derived S100 family member myeloid-related protein-14 regulates thrombosis. J Clin Invest 124:2160-71 (2014). Mouse . Read more (PubMed: 24691441) »

    See 1 Publication for this product

    Product Wall

    Thank you for your inquiry.

    We have two lots in stock currently. I don't see a recentorder from Miami so unfortuantely I can't tell you exactly which lot you would get. But the current lots we have in stock are GR76267-2 at 0.2810 mg/ml and G...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Human Tissue sections (Gastric biopsy)
    Antigen retrieval step Heat mediated
    Permeabilization No
    Specification Gastric biopsy
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative Formaldehyde
    Username

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    投稿 May 13 2016

    Application Western blot
    Sample Human Cell lysate - other (Human gingival crevicular fluid)
    Gel Running Conditions Reduced Denaturing (4-15% gel)
    Loading amount 5 µg
    Specification Human gingival crevicular fluid
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

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    投稿 Jul 24 2015

    Thank you for contacting us.

    The reducing and denaturing conditions are most commonly used for antibodies, only handful of antibodies detects the native and non-reduced protein which is why this information is not necessary to be published on ...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (human neutrophil)
    Loading amount 20 µg
    Specification human neutrophil
    Treatment 10mM FMLP for 10min
    Gel Running Conditions Reduced Denaturing (15%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    投稿 Nov 07 2012

    Thank you for contacting us.

    As both antibodies contain Glycerol, they should be quite safe from Frost/Thaw damage (as they shouldn't freeze at all). In the case of these two antibodies the aliquots are rather a protection against contaminatio...

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    Thank you for your enquiry.

    I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, any MRP8 and MRP14 antibody tested ...

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    Thank you for your inquiry.

    All of the information we have for each of our products is listed on the online datasheet for your convenience. These are updated as soon as any new information is brought to our attention. These two products ha...

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    Thank you for contacting Abcam. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which gua...

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    Thank you for contacting us.

    Yes, this antibody ab92331 has been tested in IHC-P as well as human samples. We do guarantee the antibody for this application and species.

    We guarantee all our products for TESTED species and applicat...

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