製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to MRP5
  • 特異性
    This antibody is expected to recognize both reported isoforms (NP_005679.2; NP_001018881.1) of MRP5.
  • アプリケーション
    適用あり: IHC-P, ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken, Cow, Dog
  • 免疫原

    Synthetic peptide:

    KDIDIGKEYIIP-C

    from the N terminus of the human MRP5 protein sequence according to NP_005679.2; NP_001018881.1.

  • ポジティブ・コントロール
    • Human brain (frontal cortex) and mouse brain lysates

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab77369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 4 - 6 µg/ml.
ELISA 1/32000. This antibody has only been tested in peptide ELISA.
WB Use a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 161 kDa.

ターゲット情報

  • 機能
    Acts as a multispecific organic anion pump which can transport nucleotide analogs.
  • 配列類似性
    Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ABC 33 antibody
    • ABC33 antibody
    • ABCC 5 antibody
    • Abcc5 antibody
    • ATP binding cassette sub family C (CFTR/MRP) member 5 antibody
    • ATP binding cassette sub family C member 5 antibody
    • ATP-binding cassette sub-family C member 5 antibody
    • Canalicular multispecific organic anion transporter C antibody
    • DKFZp686C1782 antibody
    • EST277145 antibody
    • MOAT C antibody
    • MOAT-C antibody
    • MOATC antibody
    • MRP 5 antibody
    • MRP5_HUMAN antibody
    • Multi specific organic anion tranporter C antibody
    • Multi-specific organic anion transporter C antibody
    • Multidrug resistance associated protein 5 antibody
    • Multidrug resistance-associated protein 5 antibody
    • pABC 11 antibody
    • pABC11 antibody
    • SMRP antibody
    see all

画像

  • Anti-MRP5 antibody (ab77369) at 0.5 µg/ml + human frontal cortex lysate in RIPA buffer at 35 µg

    Predicted band size : 161 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
  • ab77369 at 4 µg/ml staining MRP5 in Human Kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed, with detection by HRP-staining. Similar results were found with antigen retrieval at pH9.

参考文献

ab77369 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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