製品の概要
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製品名Anti-MRP4 antibody [M4I-10]
MRP4 一次抗体 製品一覧 -
製品の詳細Rat monoclonal [M4I-10] to MRP4
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由来種Rat
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アプリケーション適用あり: IHC-P, ICC/IF, WB, IHC-Fr, ICCmore details
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種交差性交差種: Mouse, Human
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免疫原
This antibody was selected after immunization with a fusion protein containing the E. coli maltose binding protein and a fragment of the human MRP4 protein corresponding to amino acids 372-431.
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ポジティブ･コントロール
- Kidney tissue This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.1% Sodium Azide
Constituents: 0.7% BSA, Tissue culture supernatant -
精製度Tissue culture supernatant
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ポリ/モノモノクローナル
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クローン名M4I-10
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アイソタイプIgG2a
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab15602 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 22272278PFA fixed cells | |
| WB | 1/20 - 1/50. Predicted molecular weight: 159 kDa. | |
| IHC-Fr | 1/20. Fix with acetone. | |
| ICC | 1/20 - 1/50. (acetone fixed cytospin preparations) |
ターゲット情報
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機能May be an organic anion pump relevant to cellular detoxification.
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組織特異性Widely expressed, with particularly high levels in prostate, but is barely detectable in liver.
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配列類似性Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains. -
細胞内局在Membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 10257 Human
- Entrez Gene: 239273 Mouse
- Omim: 605250 Human
- SwissProt: O15439 Human
- Unigene: 508423 Human
- Unigene: 40537 Mouse
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別名
- ABCC 4 antibody
- ABCC4 antibody
- ATP binding cassette sub family C (CFTR/MRP) member 4 antibody
see all
画像
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![Western blot - Anti-MRP4 antibody [M4I-10] (ab15602)](http://a.abcam.com/ps/products/15/ab15602/Images/ab15602-312090-ab15602KOWBMPES.jpg)
Western blot - Anti-MRP4 antibody [M4I-10] (ab15602)All lanes : Anti-MRP4 antibody [M4I-10] (ab15602) at 20 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : ABCC4 (MRP4) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 159 kDaLanes 1 - 2: Merged signal (red and green). Green - ab15602 observed at 200-250 kDa. Red - loading control, ab176560, observed at 50 kDa.
ab15602 was shown to specifically react with MRP4 in wild-type HAP1 cells as signal was lost in ABCC4 (MRP4) knockout cells. Wild-type and ABCC4 (MRP4) knockout samples were subjected to SDS-PAGE. Ab15602 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 20 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rat IgG H&L (IRDye® 800CW) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [M4I-10] (ab15602)](http://a.abcam.com/ps/products/15/ab15602/Images/ab15602-196912-ab15602918181edited.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [M4I-10] (ab15602)HC image of MRP4 staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab15602, 10µg/ml, for 15 mins at room temperature. A Goat anti-Rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Immunocytochemistry/ Immunofluorescence - Anti-MRP4 antibody [M4I-10] (ab15602)](http://a.abcam.com/ps/products/15/ab15602/Images/MRP4-Primary-antibodies-ab15602-4.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MRP4 antibody [M4I-10] (ab15602)Image from Juuti-Uusitalo K et al., PLoS One. 2012;7(1):e30089. Epub 2012 Jan 17.Fig 4.; doi:10.1371/journal.pone.0030089; January 17, 2012, PLoS ONE 7(1): e30089.ab15602 staining MRP4 (green) in early cobblestone Human ESC-RPEs by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with paraformaldehyde and blocked with 3% BSA in PBS for 1 hour at room temperature; antigen retrieval was by 0.1% Triton X-100/PBS. Samples were incubated with primary antibody (1/100 in 0.5% BSA-PBS) for 2 hours at room temperature. An AlexaFluor®488-conjugated goat anti-rabbit IgG (1/1500) was used as the secondary antibody.
プロトコール
参考文献
This product has been referenced in:
- Sekhar GN et al. Organic cation transporter 1 (OCT1) is involved in pentamidine transport at the human and mouse blood-brain barrier (BBB). PLoS One 12:e0173474 (2017). Read more (PubMed: 28362799) »
- Chen JJ et al. MRP4 regulates ENaC-dependent CREB/COX-2/PGE2signaling during embryo implantation. Oncotarget 8:78520-78529 (2017). Read more (PubMed: 29108246) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"