Customer reviews and FAQs

製品の概要

  • 製品名
    Anti-MRP4 antibody [M4I-10]
    MRP4 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [M4I-10] to MRP4
  • アプリケーション
    適用あり: IHC-P, ICC/IF, WB, IHC-Fr, ICCmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    This antibody was selected after immunization with a fusion protein containing the E. coli maltose binding protein and a fragment of the human MRP4 protein corresponding to amino acids 372-431.

  • ポジティブ･コントロール
    • Kidney tissue This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration. PubMed: 22272278PFA fixed cells
WB 1/20 - 1/50. Predicted molecular weight: 159 kDa.
IHC-Fr 1/20. Fix with acetone.
ICC 1/20 - 1/50. (acetone fixed cytospin preparations)

ターゲット情報

  • 機能
    May be an organic anion pump relevant to cellular detoxification.
  • 組織特異性
    Widely expressed, with particularly high levels in prostate, but is barely detectable in liver.
  • 配列類似性
    Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
    • 別名
      • ABCC 4 antibody
      • ABCC4 antibody
      • ATP binding cassette sub family C (CFTR/MRP) member 4 antibody
      • ATP binding cassette sub family C member 4 antibody
      • ATP-binding cassette sub-family C member 4 antibody
      • bA464I2.1 (ATP binding cassette, sub-family C (CFTR/MRP) member 4) antibody
      • bA464I2.1 antibody
      • Canalicular multispecific organic anion transporter antibody
      • Canalicular multispecific organic anion transporter ABC superfamily antibody
      • EST170205 antibody
      • MOAT B antibody
      • MOAT-B antibody
      • MOATB antibody
      • MRP 4 antibody
      • MRP/cMOAT related ABC transporter antibody
      • MRP/cMOAT-related ABC transporter antibody
      • MRP4_HUMAN antibody
      • Multi specific organic anion transporter B antibody
      • Multi-specific organic anion transporter B antibody
      • Multidrug resistance associated protein 4 antibody
      • Multidrug resistance-associated protein 4 antibody
      • OTTHUMP00000018560 antibody
      see all

    Anti-MRP4 antibody [M4I-10] 画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [M4I-10] (ab15602)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [M4I-10] (ab15602)

      HC image of MRP4 staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab15602, 10µg/ml, for 15 mins at room temperature. A Goat anti-Rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Immunocytochemistry/ Immunofluorescence - Anti-MRP4 antibody [M4I-10] (ab15602)
      Immunocytochemistry/ Immunofluorescence - Anti-MRP4 antibody [M4I-10] (ab15602)Image from Juuti-Uusitalo K et al., PLoS One. 2012;7(1):e30089. Epub 2012 Jan 17.Fig 4.; doi:10.1371/journal.pone.0030089; January 17, 2012, PLoS ONE 7(1): e30089.
      ab15602 staining MRP4 (green) in early cobblestone Human ESC-RPEs by Immunocytochemistry/ Immunofluorescence.

      Cells were fixed with paraformaldehyde and blocked with 3% BSA in PBS for 1 hour at room temperature; antigen retrieval was by 0.1% Triton X-100/PBS. Samples were incubated with primary antibody (1/100 in 0.5% BSA-PBS) for 2 hours at room temperature. An AlexaFluor®488-conjugated goat anti-rabbit IgG (1/1500) was used as the secondary antibody.

    Anti-MRP4 antibody [M4I-10] (ab15602) 使用論文

    This product has been referenced in:
    • Rohrer PR  et al. Is nuclear factor erythroid 2-related factor 2 responsible for sex differences in susceptibility to acetaminophen-induced hepatotoxicity in mice? Drug Metab Dispos 42:1663-74 (2014). Read more (PubMed: 25092713) »
    • Steinberg F  et al. A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport. Nat Cell Biol 15:461-71 (2013). Human . Read more (PubMed: 23563491) »

    See all 12 Publications for this product

    ab15602 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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    ab15602 の abreview を投稿する ab15602 に関する問い合わせをする

    8 Abreviews or Q&A

    10
    Votes: Highest First

    Hallo, i am looking for a second antibody with FITC conjugate that fits together with ab15602 MRP4 antibody from abcam. I already tried an goat anti-rat IgG antibody from sigma and this did not worked. thank you, Jessica Verstraelen

    In order to find the secondary antibody that best fits your necessities; I would like you to provide us with further information, such as in which application are you testing the antibody or the host species for the secondary.
    However, you can als...

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    Reviewing this case, I would like to offer some suggestions to help optimise the results from these antibodies. I would also appreciate if you can confirm some further details:
    1. Please confirm the order numbers and dates of purchase.
    Order ...

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    Thank you for your reply and for kindly providing the requested details.

    I look forward to hearing from you with the results of the next experiment. i can recommend it would be beneficial to consider a reducing gel. This will ensure the prot...

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    Thank you for your time and cooperation. I look forward to receiving the completed questionnaire.
    Order Details
    Antibody code:
    MRP4: ab15602, GR79982-1
    MRP5: ab 3377, GR43649-3
    MRP2: ab 3373, GR19771-1
    Problem
    Choose:...

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    Thank you for taking the time to complete our questionnaire. The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

    Reviewing this case, I would...

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    Hallo,

    I tried to localize MRP4 and MRP5 in Caco2 cells cultivated in chamber
    slides. The second antibody was a goat anti-rat FITC labelled from
    another company. Both antibodies didin't show any fluorescence. Is it maybe possible t...

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    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from these antibodies.

    I am sorry to confirm that because we carry over 90,000 products, it is regrettably not feasible fo...

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    Do you have images of Western blots using these two MRP4 antibodies, ab15602 and ab15598?

    Thank you for your enquiry. The publication found at the following link contains images of Western blots for both of these antibodies: http://mcb.asm.org/cgi/content/full/24/17/7612?view=long&pmid=15314169 I hope this helps. Please contact us ...

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    Please recommend a positive control for WB in HEK (human embryonic kidney) cells. I will use AB ab15602 or ab15598. Thanks Could you also please advise me on a positive control for MRP5 (WB, human cells). Thanks. MRP5; ab3377

    Thank you for your enquiry. I am sorry for the delay to your enquiry; I have been in touch with the source of this antibody and a response has not been forthcoming. For the testing of antibodies ab15598 and ab15602 against MRP4 I have been informed...

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    We have a customer calling about one of your products ab15602. Customer was doing testing on Western Blot. The test methods he used are as follows: 100 µg of b16 cancer cell lysate Customer used both 1% milk and also used 1% BSA Primary Ab d...

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    Thank you for contacting us for technical support with ab15602. I am currently trying to find out the recommended positive control for this antibody and as soon as I have this information I will let you know. The antibody is supplied as tissue cult...

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    I have questions for Ab 15602 Please could you give me informations : have you heard about cross reactivity with the rat MARP4 with this Ab ? Is there any unspecificity problems on detecting this protein on tha rat ? as seen on a recent paper : molec...

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    Thank you for your enquiry regarding ab15602. This antibody has not been tested in rat (only human and mouse) but we would not advise to use it in rat in IHC as the antibody has been raised in rat and hence the secondary antibody anti-rat is very like...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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