Anti-MRP2 抗体 [M2III-5] (ab15603)
Key features and details
- Mouse monoclonal [M2III-5] to MRP2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-MRP2 antibody [M2III-5]
MRP2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [M2III-5] to MRP2 -
由来種
Mouse -
特異性
This antibody does not cross-react with the human MDR1 P-gp, MRP1, MRP3, or MRP5 gene products. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
非交差種: Mouse -
免疫原
This antibody was raised against a fusion protein of the bacterial maltose binding protein and rat MRP2, containing the 202-amino acid C terminal end of the transporter protein.
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エピトープ
ab15603 reacts with an internal epitope of MRP2; a 190-200 kD transmembrane protein earlier known as the canalicular multi-organic anion transporter cMOAT, absent in patients with the Dubin-Johnson syndrome. -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.3
Preservative: 0.1% Sodium azide
Constituents: Tissue culture supernatant, 0.7% BSA -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
M2III-5 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab15603の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/20 - 1/50. Predicted molecular weight: 185.4 kDa.
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特記事項 |
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WB
1/20 - 1/50. Predicted molecular weight: 185.4 kDa. |
ターゲット情報
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機能
Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter. -
組織特異性
Found on the apical membrane of polarized cells in liver, kidney and intestine. The highest expression is found in liver. -
関連疾患
Defects in ABCC2 are the cause of Dubin-Johnson syndrome (DJS) [MIM:237500]. DJS is an autosomal recessive disorder characterized by conjugated hyperbilirubinemia, an increase in the urinary excretion of coproporphyrin isomer I, deposition of melanin-like pigment in hepatocytes, and prolonged retention of sulfobromophthalein, but otherwise normal liver function. -
配列類似性
Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1244 Human
- Omim: 601107 Human
- SwissProt: Q92887 Human
- Unigene: 368243 Human
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別名
- ABC30 antibody
- abcC2 antibody
- ATP binding cassette sub family C (CFTR/MRP) member 2 antibody
see all
画像
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All lanes : Anti-MRP2 antibody [M2III-5] (ab15603) at 1/20 dilution
Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : ABCC2 knockout A549 (Human lung carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 185.4 kDaLanes 1 - 2: Merged signal (red and green). Green - ab15603 observed at 210 kDa. Red - loading control, ab52866, observed at 50 kDa.
ab15603 was shown to recognize ABCC2 in wild-type A549 cells as signal was lost at the expected MW in ABCC2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ABCC2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab15603 and ab52866 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/20 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab15603 は 13 報の論文で使用されています。
- Chen SL et al. Investigation of the role of the autophagic protein LC3B in the regulation of human airway epithelium cell differentiation in COPD using a biomimetic model. Mater Today Bio 13:100182 (2022). PubMed: 34917923
- Roopesh RP et al. High-throughput production of liver parenchymal microtissues and enrichment of organ-specific functions in gelatin methacrylamide microenvironment. Biotechnol Bioeng 119:1018-1032 (2022). PubMed: 34931302
- Okamoto K et al. Antioxidant effect of ascorbic acid against cisplatin-induced nephrotoxicity and P-glycoprotein expression in rats. Eur J Pharmacol 909:174395 (2021). PubMed: 34332922
- Yang AJ et al. Acute exercise and brain BACE1 protein content: a time course study. Physiol Rep 7:e14084 (2019). PubMed: 31033251
- Baranowski BJ et al. Evaluation of neuropathological effects of a high-fat high-sucrose diet in middle-aged male C57BL6/J mice. Physiol Rep 6:e13729 (2018). PubMed: 29890051