製品の概要

  • 製品名Anti-MRP1 antibody [MRPm5]
    MRP1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MRPm5] to MRP1
  • 特異性MRPm5 does not cross-react with the human MDR1 and MDR3 gene products.
  • アプリケーション適用あり: IHC-Fr, IHC-P, Flow Cyt, WB, ICCmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Fusion protein, corresponding to amino acids 986-1204 of Human MRP1.

  • エピトープMRP1 antibody reacts with an internal epitope of MRP1.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab24102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/20.
IHC-P 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Perform heat mediated antigen retrieval in citrate buffer (pH 6.0) for 10 minutes before commencing with IHC staining protocol.

Flow Cyt 1/20 - 1/50. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB 1/20 - 1/50. Predicted molecular weight: 190 kDa.
ICC 1/20 - 1/50.

ターゲット情報

  • 機能Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency.
  • 組織特異性Lung, testis and peripheral blood mononuclear cells.
  • 配列類似性Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ABC 29 antibody
    • ABC29 antibody
    • ABCC 1 antibody
    • ABCC antibody
    • Abcc1 antibody
    • ATP binding cassette sub family C (CFTR/MRP) member 1 antibody
    • ATP binding cassette sub-family C member 1 antibody
    • ATP-binding cassette sub-family C member 1 antibody
    • DKFZp686N04233 antibody
    • DKFZp781G125 antibody
    • GS X antibody
    • GSX antibody
    • Leukotriene C(4) transporter antibody
    • LTC4 transporter antibody
    • MRP 1 antibody
    • MRP antibody
    • MRP1_HUMAN antibody
    • Multidrug resistance associated protein 1 antibody
    • Multidrug resistance protein antibody
    • Multidrug resistance-associated protein 1 antibody
    • Multiple drug resistance associated protein antibody
    • Multiple drug resistance protein 1 antibody
    see all

Anti-MRP1 antibody [MRPm5] 画像

  • ICC/IF image of Anti-MRP1 antibody (ab24102) stained human ES cell derived hepatocytes. The cells were fixed in methanol, blocked and permeabilized using 10% BSA in PBS/0.1% Tween, for 1 hour at 20oC. The cells were then incubated with ab24102 at a 1/50 dilution for 16 hours at 4oC. The secondary antibody was a Donkey anti-Mouse Alexa Fluor 488, used at a 1/400 dilution.

    See Abreview

  • Overlay histogram showing HL60 cells stained with ab24102 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24102, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-MRP1 antibody [MRPm5] (ab24102) 使用論文

This product has been referenced in:
  • Ji L  et al. Correlation of Nrf2, NQO1, MRP1, cmyc and p53 in colorectal cancer and their relationships to clinicopathologic features and survival. Int J Clin Exp Pathol 7:1124-31 (2014). IHC-P ; Human . Read more (PubMed: 24695690) »
  • Helms HC  et al. An electrically tight in vitro blood-brain barrier model displays net brain-to-blood efflux of substrates for the ABC transporters, P-gp, Bcrp and Mrp-1. AAPS J 16:1046-55 (2014). Read more (PubMed: 24934296) »

See all 7 Publications for this product

Product Wall

I'm assuming you're referring to a lysis buffer for sample preparation prior to western blot. If this is the case, since MRP1 is a membrane bound protein we recommend using RIPA buffer for lysis. Our 10X RIPA buffer ab156034 is through the link below:<...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Human Cell (ES - derived hepatocytes)
Specification ES - derived hepatocytes
Permeabilization Yes - During blocking step, BSA is diluted in PBS/0.1% Tween.
Fixative Methanol
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Abcam user community

Verified customer

投稿 Aug 23 2013

Thank you for contacting us.

I do hope the suggestions made today shed some light upon the issue you are experiencing with ab24102 Anti-MRP1 antibody [MRPm5] and look forward to hear the results.

This product is covered by our Abpro...

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Thank you for your inquiry. I am happy to confirm that all these products are suitable for WB and tested and guaranteed.
I suggest ab29889 (Liver (Human) Tissue Lysate - adult normal tissue) as positive control for ab3366, ab3373, ab3375 and ab241...

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Thank you for your enquiry and your interest in our products.

Here are a few antibodies against the following targets which have been tested and characterized for Flow cytometry. Please take a look at the on-line datasheets for further inform...

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Thank you for contacting us. We do have products that are conjugated to match your needs, Ab81424 Anti-CD44 antibody [MEM-263] is conjugated with APC. Ab58754 Anti-CD44 antibody [MEM-263] (Phycoerythrin). I've included links to each below: http://www.a...

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Thank you for contacting Abcam. We offer a number of PGP antibodies that are guaranteed to work in Flow Cytometry on dog cells. I would recommend http://www.abcam.com/CD44-antibody-IM7-ab119863.htmlor the FITC conjugated http://www.abcam.com/CD44-antib...

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Thank you for contacting us. ab3364; anti P Glycoprotein is no longer available in our catalogue. However we have similar products available to buy. The catalogue numbers are ab3083; ab3366; ab10333; ab80594; ab80626. Please check the da...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Liver)
Gel Running Conditions Reduced Denaturing (Samples were not denatured prior to gel loading)
Loading amount 80 µg
Specification Liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

投稿 Feb 14 2011

The only recommendation I have seen for a western blot positive control is a lysate from cells transfected with MRP1. We do not have such a lysate or a recombinant protein. As I mentioned in our phone call, endogenous sources include lung, testis, and ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"