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Fusion protein, corresponding to amino acids 986-1204 of Human MRP1.
Our Abpromise guarantee covers the use of ab24102 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform heat mediated antigen retrieval in citrate buffer (pH 6.0) for 10 minutes before commencing with IHC staining protocol.
|Flow Cyt||1/20 - 1/50. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|WB||1/20 - 1/50. Predicted molecular weight: 190 kDa.|
|ICC||1/20 - 1/50.|
ICC/IF image of Anti-MRP1 antibody (ab24102) stained human ES cell derived hepatocytes. The cells were fixed in methanol, blocked and permeabilized using 10% BSA in PBS/0.1% Tween, for 1 hour at 20oC. The cells were then incubated with ab24102 at a 1/50 dilution for 16 hours at 4oC. The secondary antibody was a Donkey anti-Mouse Alexa Fluor 488, used at a 1/400 dilution.