製品の概要

  • 製品名
    Anti-MRGX1 antibody
  • 製品の詳細
    Rabbit polyclonal to MRGX1
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide derived from internal sequence of human GPCR MRGX1.

  • ポジティブ･コントロール
    • Extracts from MCF-7 cells. HepG2 cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab77519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 36 kDa.
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

ターゲット情報

  • 関連性
    MRGX1 is an opiod receptor that has been reported exclusively in dorsal root ganglion. MRGX1 is probably involved in the function of nociceptive neurons and may regulate nociceptor function and/or development, including the sensation or modulation of pain. It is potently activated by enkephalins including BAM22 (bovine adrenal medulla peptide 22) and BAM (8-22). BAM22 is the most potent compound and evoked a large and dose-dependent release of intracellular calcium in stably transfected cells.
  • 細胞内局在
    Cell Membrane; Multi-pass membrane protein.
  • 参照データベース
    • 別名
      • GPCR antibody
      • MAS related gene MRGX1 antibody
      • MAS related GPR member X1 antibody
      • MRGPRX1 antibody
      • MRGX1 antibody
      • SNSR4 antibody
      see all

    画像

    • Western blot - GPCR MRGX1 antibody (ab77519)
      Western blot - GPCR MRGX1 antibody (ab77519)
      All lanes : Anti-MRGX1 antibody (ab77519) at 1/500 dilution

      Lane 1 : Extracts from MCF-7 cells
      Lane 2 : Extracts from MCF-7 cells with immunising peptide at 5 µg

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 36 kDa
      Observed band size: 36 kDa

    • Immunocytochemistry/ Immunofluorescence - GPCR MRGX1 antibody (ab77519)
      Immunocytochemistry/ Immunofluorescence - GPCR MRGX1 antibody (ab77519)
      Immunofluorescence analysis of GPCR MRGX1 in HepG2 cells using ab77519, at 1/500 dilution, in the absence or presence of immunising peptide

    参考文献

    ab77519 has not yet been referenced specifically in any publications.

    ab77519 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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