Anti-Mre11 抗体 [12D7] (ab214)

Anti-Mre11 antibody [12D7] 画像

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  Western blot - Anti-Mre11 antibody [12D7] (ab214)
  
  Developed using the ECL technique
  
  Predicted band size : 79 kDa
  

  Western blot of PC12 (brain)whole cell extracts at 30µg per lane, labeling Mre11 with ab214 at 1:500. A HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

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  Western blot - Anti-Mre11 antibody [12D7] (ab214)
  All lanes : Anti-Mre11 antibody [12D7] (ab214) at 1/1000 dilution
  
  Lane 1 : 293T whole cell extract
  Lane 2 : Transfected 293T whole cell extract
  
  Lysates/proteins at 30 µg per lane.
  
  
  Predicted band size : 79 kDa
  
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  Immunohistochemistry (Frozen sections) - Anti-Mre11 [12D7] antibody (ab214)This image is courtesy of an anonymous Abreview

  ab214 staining Mre11 in human normal and gastric cancer tissue sections (20µm) by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde for 3 hours, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 1 hour at 23°C. Samples were incubated with primary antibody (1/200 in 3% BSA + 0.1% Triton X-100 in TBS buffer) for 12 hours at 4°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

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  Western blot - Anti-Mre11 [12D7] antibody (ab214)This image is courtesy of an anonymous Abreview
  All lanes : Anti-Mre11 antibody [12D7] (ab214) at 1/500 dilution
  
  Lane 1 : Mouse breast cancer cell line - whole cell lysate. Transfected with vector control.
  Lane 2 : Mouse breast cancer cell line - whole cell lysate. Transfected with knockdown shRNA targeting Mre11 gene.
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  HRP-conjugated Goat anti-mouse IgG polyclonal at 1/1000 dilution
  Developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 79 kDa
  Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  
  
  Exposure time : 1 minute

  This image is courtesy of an anonymous Abreview

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  Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] (ab214)Image supplied by Dr Domenico Delia, Istituto Nazionale Tumori, Italy.

  Indirect immunofluorescence to detect localisation of Mre11 in normal lymphoblastoid cells.
  (Panel D - negative control).

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  Western blot - Anti-Mre11 antibody [12D7] (ab214)
  
  
  Predicted band size : 79 kDa
  

  This picture was kindly supplied as part of the reviews for ab214 and for ab89 submitted by Anya Polischouk.

  The bands represent nuclear (N) and cytoplasmic (C) extract from human lung cancer cells (U1810).

   

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mre11 antibody [12D7] (ab214)This image is courtesy of an anonymous Abreview
  ab214 at 1/200 staining normal human bronchus tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in EDTA was peformed. The tissue was blocked in normal rabbit serum and then incubated with the antibody for 1 hour. An HRP conjugated goat polyclonal antibody was used as the secondary.

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  Flow Cytometry - Anti-Mre11 antibody [12D7] (ab214)

  Overlay histogram showing HeLa cells stained with ab214 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab214, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG, H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.