The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: 1-4 µg/mg lysate.
WB: 1/2500 - 1/25000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint.
Present in rapidly proliferating cell lines.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.