The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報IP: 1-4 µg/mg lysate.
WB: 1/2500 - 1/25000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint.
組織特異性Present in rapidly proliferating cell lines.
配列類似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.