製品の概要

  • 製品名Anti-MPRA antibody
    MPRA 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MPRA
  • 特異性This antibody detects endogenous levels of total MPRA protein.
  • アプリケーション適用あり: ICC, ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A synthesized peptide derived from an internal region of human MPRA.

  • ポジティブ・コントロール
    • Extracts from 293 and COLO cells

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab75508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent concentration.
ELISA 1/5000.
WB 1/500 - 1/1000. Predicted molecular weight: 40 kDa.

ターゲット情報

  • 関連性MPRA belongs to the ADIPOR family. It is a steroid membrane receptor which binds progesterone in vitro. MPRA may be involved in oocyte maturation. It is expressed in ovary, testis, placenta, uterus and bladder
  • 細胞内局在Membrane; Multi pass membrane protein
  • 参照データベース
  • 別名
    • Membrane progestin receptor alpha antibody
    • mPR alpha antibody
    • MRPA antibody
    • mSR antibody
    • PAQR7 antibody
    • PGLP antibody
    • Progestin and adipoQ receptor family member VII antibody
    see all

Anti-MPRA antibody 画像

  • All lanes : Anti-MPRA antibody (ab75508) at 1/500 dilution

    Lane 1 : Extracts from COLO cells
    Lane 2 : Extracts from 293 cells
    Lane 3 : Extracts from 293 cells with immunizing peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 40 kDa
    Observed band size : 40 kDa
  • ab75508 staining MPRA in murine ovary tissue sections by Immunohistochemistry (PFA-fixed).
    Tissue was fixed in paraformaldehyde, blocked using 10% normal goat serum for 1 hour at 25°C and then incubated with ab75508 at a 1/500 dilution for 12 hours at 4°C. The secondary used was a biotin conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.

    Procedures were tested with and without a permeabilization step and better results were acquired by eliminating the permeabilization step, due to PAQR7 being expressed on the cell membrane.

    See Abreview

Anti-MPRA antibody (ab75508) 使用論文

This product has been referenced in:
  • Bashour NM & Wray S Progesterone directly and rapidly inhibits GnRH neuronal activity via progesterone receptor membrane component 1. Endocrinology 153:4457-69 (2012). Read more (PubMed: 22822163) »

See 1 Publication for this product

Product Wall



It is catalog number ab171582.

Application Immunocytochemistry
Sample Mouse Cell (Mouse ovary sections)
Specification Mouse ovary sections
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Mr. Nicholas Bashour

Verified customer

投稿 Dec 07 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"