製品の概要

  • 製品名
    Anti-MPPE1 antibody
  • 製品の詳細
    Rabbit polyclonal to MPPE1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 108-157 (LLQPEVVFIL GDIFDEGKWS TPEAWADDVE RFQKMFRHPS HVQLKVVAGN) of Human MPPE1 (NP_075563).

  • ポジティブ・コントロール
    • Transfected 293T cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab87298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 関連性
    The specific function of metallophosphoesterase 1 (MPPE1) is not yet known. There are 5 isoforms produced by alternative splicing which have molecular weights of 45 kDa, 39 kDa (2 isoforms), 38 kDa and 29 kDa.
  • 細胞内局在
    Endoplasmic reticulum-Golgi intermediate compartment membrane; Multi-pass membrane protein. Golgi apparatus, cis-Golgi network membrane; Multi-pass membrane protein. Note: Also localizes to endoplasmic reticulum exit site.
  • 参照データベース
  • 別名
    • EC 3.1.-.- antibody
    • Metallo phosphoesterase antibody
    • Metallophosphoesterase 1 antibody
    • PGAP5 antibody
    • Post-GPI attachment to proteins factor 5 antibody
    see all

画像

  • Anti-MPPE1 antibody (ab87298) at 1 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 45 kDa
    Observed band size : ~40 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab87298 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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