The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
The specific function of metallophosphoesterase 1 (MPPE1) is not yet known. There are 5 isoforms produced by alternative splicing which have molecular weights of 45 kDa, 39 kDa (2 isoforms), 38 kDa and 29 kDa.