製品の概要

  • 製品名Anti-MPP8 antibody
    MPP8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MPP8
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Cow, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide within Human MPP8 aa 400-500 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q99549

  • ポジティブ・コントロール
    • This antibody gave a positive signal in MCF7 Nuclear and HEK293 whole cell lysates.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab124499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 97 kDa).

ターゲット情報

  • 配列類似性Contains 4 ANK repeats.
    Contains 1 chromo domain.
  • 翻訳後修飾Phosphorylated in M (mitotic) phase.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HSMPP8 antibody
    • M-phase phosphoprotein 8 antibody
    • M-phase phosphoprotein, mpp antibody
    • M-phase phosphoprotein, mpp8 antibody
    • MPHOSPH8 antibody
    • MPP8_HUMAN antibody
    • RP11-523H24.1 antibody
    • Twa3 antibody
    • Two hybrid-associated protein 3 with RanBPM antibody
    see all

Anti-MPP8 antibody 画像

  • All lanes : Anti-MPP8 antibody (ab124499) at 1 µg/ml

    Lane 1 : MCF7 nuclear extract lysate (ab14860)
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 97 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 80 kDa (possible non-specific binding).

    Exposure time : 2 minutes

    The predicted molecular weight of MPP8 is 97 kDa (SwissProt), however we expect to observe a banding pattern around 125 kDa.

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab124499 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

Anti-MPP8 antibody (ab124499) 使用論文

ab124499 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab124499.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"