製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MPP8
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Cow, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide within Human MPP8 aa 400-500 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q99549

  • ポジティブ・コントロール
    • This antibody gave a positive signal in MCF7 Nuclear and HEK293 whole cell lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab124499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 97 kDa).

ターゲット情報

  • 配列類似性
    Contains 4 ANK repeats.
    Contains 1 chromo domain.
  • 翻訳後修飾
    Phosphorylated in M (mitotic) phase.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HSMPP8 antibody
    • M-phase phosphoprotein 8 antibody
    • M-phase phosphoprotein, mpp antibody
    • M-phase phosphoprotein, mpp8 antibody
    • MPHOSPH8 antibody
    • MPP8_HUMAN antibody
    • RP11-523H24.1 antibody
    • Twa3 antibody
    • Two hybrid-associated protein 3 with RanBPM antibody
    see all

画像

  • All lanes : Anti-MPP8 antibody (ab124499) at 1 µg/ml

    Lane 1 : MCF7 nuclear extract lysate (ab14860)
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 97 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 80 kDa (possible non-specific binding).

    Exposure time : 2 minutes

    The predicted molecular weight of MPP8 is 97 kDa (SwissProt), however we expect to observe a banding pattern around 125 kDa.

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab124499 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

プロトコール

参考文献

ab124499 has not yet been referenced specifically in any publications.

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