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Our Abpromise guarantee covers the use of ab75895 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 28 kDa.Can be blocked with MOX1 peptide (ab199249).|
|ICC/IF||1/10 - 1/50.|
Immunofluorescence of HeLa cells labelling MOX1 with ab75895. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab75895 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃) and cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). MOX1 immunoreactivity is localised to the nucleolus.
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